双醋瑞因对碘乙酸钠诱导的骨关节炎软骨细胞损伤的保护作用

Protective effect of diacerein on MIA-induced injury in rat osteoarthritis chondrocytes

目的探讨双醋瑞因对碘乙酸钠(MIA)诱导的骨关节炎软骨细胞的保护作用。方法实验分为正常组,模型组(4μM MIA),双醋瑞因低、中、高剂量组(1、10、100μM)。噻唑蓝(MTT)法检测各组软骨细胞活力;分光光度法检测各组半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)活性;Western blot分析核因子-κB(NF-κB)信号通路激活情况及下游靶蛋白Bax、Bcl-2、基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-13(MMP-13)的表达量。结果 1、10、100μM双醋瑞因能提高MIA诱导的大鼠软骨细胞的活力并降低Caspase-3活性(P<0.05);10、100μM双醋瑞因能降低IκBα及NF-κB磷酸化水平,并下调Bax、MMP-9及MMP-13的表达,上调Bcl-2的表达(P<0.05)。结论双醋瑞因能够抑制MIA诱导的软骨细胞凋亡与细胞外基质降解,与NF-κB信号通路有关。

Objective To investigate the protective effect of diacerein on monosodium iodoacetate（MIA）induced injury in rat osteoarthritis chondrocytes.Methods The experiment was divided into five groups,including the normal group,model group（4μM MIA）,diacerein low,middle and high doses groups（1,10,100μM）.The viability of chondrocytes was detected by MTT assay.The activity of cysteinyl aspartate specific proteinase-3（Caspase-3）was measured by spectrophotography.The activation of nuclear factor kappa B（NF-κB）signaling pathway and expression level of downstream target molecule cell Bax,Bcl-2,matrix metalloproteinase-9（MMP-9）and MMP-13 were detected by Western blot.Results 1,10,100μM diacerein could increase the viability of MIAinduced chondrocytes and reduce the activity of Caspase-3（P〈0.05）.10,100μM diacerein could decrease the phosphorylation level of IκBαand NF-κB p65,furthermore downregulated the level of Bax,MMP-9and MMP-13 protein,and upregulated the level of Bcl-2protein（P〈0.05）.Conclusion Diacerein could inhibit cell apoptosis and degradation of extracellular matrix in MIA-induced rat chondrocytes,which might be related to the NF-κB signal pathway.