雷帕霉素增加耐药胃癌细胞对多柔比星的敏感性

Rapamycin increases the sensitivity of doxorubicin-resistant gastric cancer cell to doxorubicin

目的探讨雷帕霉素对耐药胃癌细胞对多柔比星敏感性的影响。方法用含低浓度(0.5μmol/L)多柔比星培养基培养并建立耐药胃癌细胞株MKN45-R和SNU1-R,以不同浓度的多柔比星处理胃癌细胞48h后,用CCK-8试剂盒测得细胞活力,计算半数抑制浓度(IC_(50))。采用10μmol/L雷帕霉素预处理耐多柔比星胃癌细胞后再次测定IC_(50)。结果多柔比星对胃癌细胞株MKN45和SNU1的生长有剂量依赖性抑制作用,IC_(50)分别为(57.2±4.0)μmol/L和(49.9±4.7)μmol/L,耐药细胞株MKN45-R和SNU1-R的多柔比星IC_(50)显著增大,分别为(153.7±6.7)μmol/L和(172.8±13.1)μmol/L。低浓度雷帕霉素可增加耐药细胞MKN45-R和SNU1-R对多柔比星的敏感性,IC_(50)显著减小为(69.9±2.3)μmol/L和(50.0±1.7)μmol/L。结论雷帕霉素可增加耐药胃癌细胞对多柔比星的敏感性。

Objective To investigate the effect of rapamycin on the sensitivity of doxorubicinresistant gastric cancer cell to doxorubicin.Methods The doxorubicin-resistant gastric cancer cell lines MKN45-R and SNU1-R were obtained by exposing to the culture media containing lowconcentration（0.5μmol/L）doxorubicin.The viability of gastric cancer cell was tested with CCK-8kit and the harf inhibitory concentration（IC_（50））of doxorubicin was calculated.Results Doxorubicin inhibited the proliferation of MKN45 and SNU1in a dose-dependent manner,with IC_（50）（57.2±4.0）μmol/L and（49.9±4.7）μmol/L,respectively.Rapamycin in lower dose increased the sensitivity of doxorubicin-resistant gastric cancer cell MKN45-R and SNU1-R to doxorubicin with a significant lower IC_（50）[（69.9±2.3）μmol/L vs.（153.7±6.7）μmol/L and（50.0±1.7）μmol/L vs.（172.8±13.1）μmol/L）.Conclusion Rapamycin increases the sensitivity of doxorubicin-resistant gastric cancer cell to doxorubicin.