银离子对卡介苗菌株黏附硅胶管表面特性的影响

Impact of silver ions on features of BCG′ s adhesion on the silicone tube surface

目的:研究银离子对卡介苗黏附硅胶管表面能力的影响。方法:分别将普通硅胶管与镀银硅胶管在卡介苗菌液中培养30 d,每3 d测定菌液OD值,绘制生长曲线;依据卡介苗分裂周期,在分裂不同代数时取出硅胶管并洗脱,于固体培养基上接种观察和记录查看菌落数量,绘制黏附曲线;电镜观察培养30d的硅胶管细菌黏附情况。结果 :镀银硅胶管组每次采样测定的OD值和菌斑个数均少于普通硅胶管组,差异具有统计学意义(P<0.05)。电镜观察镀银硅胶管组卡介苗黏附数量也少于普通硅胶管组。结论:银离子可降低卡介苗菌株在硅胶管表面的黏附并且可抑制卡介苗的生长。

Objective To explore the impact of silver ions on the adhesion ability of Bacillus Calmette-Gueri vaccine on silicone tube surface. Methods Common and silver plated silicone tubes were cultured in BCG bacterial liquid for 30 days. A gas-bacilli was taken once every 3 days to measure OD value of microbial. The growth curve of BCG vaccine was made. The corresponding silicone tubes in divided different generations was removed and eluted based on the division cycle of BCG vaccine. The samples were inoculated on the solid medium for observing the colony growth amount, and adhesion curve was drawn. The silicone tube colony line was observed under the electron microscope at day 30. Results OD value and bacterial plaque number were less in silver silicone tube group than in common silicone tube group, with statistical significances （P 〈 0.05）. Electron microscopy also showed that BCG adhesion on silver silicone tube group was relatively less than that on common silicone tube group. Conclusion Silver ions can reduce the adhesion of BCG vaccine on silicone tube surface and inhibit the growth of BCG vaccine.