广西巴马地区长寿人群沉默信号调节蛋白6基因mRNA的表达

mRNA expression of silent information regulator 6 among population in Bama county of Guangxi

目的：分析广西巴马有长寿家族史人群外周血沉默信号调节蛋白6（SIRT6）基因的mRNA表达水平,探讨其与SIRT6基因多态性及其蛋白的关系。方法：选取广西巴马地区有长寿家族史者（长寿家族史组）137例（年龄30~106岁,男70例,女67例,汉族占6.57%,壮族和瑶族占93.43%）,无长寿家族史者（无长寿家族史组）91例（年龄22~89岁,男51例,女40例,均为壮族和瑶族）。采用实时荧光定量RT-PCR方法检测两组人群外周血SIRT6基因mRNA的表达水平。结果：长寿家族史组总体和女性人群外周血SIRT6 mRNA表达水平均高于无长寿家族史组（P〈0.05）。长寿家族史组SIRT6基因rs350846位点GG、CG基因型及G等位基因携带者（GG、CG）的SIRT6 mRNA水平均高于无长寿家族史组（P〈0.05）。长寿家族史组SIRT6 mRNA表达水平与血清中SIRT6蛋白无相关关系（P〉0.05）。结论：SIRT6基因mRNA表达水平与广西巴马人群家庭聚集性长寿相关,且G等位基因携带者的SIRT6 mRNA高表达更有利于长寿。

Objective To analyze mRNA expression of silent information regulator 6 （SIRT6） gene in .the blood of population with family history of longevity in Bama county of Guangxi and to explore its association with SIRT6 gene polymorphism and its protein Methods One hundred and thirty-seven people （aged 30 - 106, 70 males, 67 females, 6.57% Han nationality, 93.43% Zhuang and Yao nationalities） with family history of longevity （long-lived family history group）, and 91 people （aged 22 - 89, 51 males, 40 females, all Zhuang and Yao nationalities） without family history of longevity were recruited in the study （non-long-lived family history group）. Real-time fluorescence quantitative RT-PCR was used to detect mRNA expression of SIRT6 gene in two groups. Results SIRT6 mRNA expression of total and femals in long-lived family history group were higher than those in non-long-lived family history group （P 〈 0.05）. mRNA expression of GG and CG genotype of SIRT6 （rs350846） and G allele carriers in long-lived family history group were all higher than those in non-long-lived family history group and the difference was statistically significant （P 〈 0.05）. SIRT6 mRNA expression was not associated with serum SIRT6 protein in long-lived family history group （P 〉 0.05）. Conclusion The expression of mRNA in SIRT6 gene may be associated with familial aggregation of longevity in Bama County of Guangxi and high expression of mRNA of SIRT6 in G allele carriers contributes to longevity.