回神颗粒对中度创伤性脑损伤大鼠神经行为学及体重的影响

Research of Huishen Granule Influence on Neurological Severity Score and Weight on Moderate Traumatic Brain Injury Model Rats

目的:通过神经损伤评分(NSS)评分和体重测量,探讨回神颗粒减轻TBI的作用机制。方法:应用液压性脑损伤装置建立中度TBI模型,创伤压力为(170±10)k Pa,作用时间为(20±2)ms,随机将大鼠分为6组:正常组、假手术组、模型3 d组、模型7 d组、治疗3 d组、治疗7 d组。治疗3 d组和治疗7 d组于创伤后即刻用2 m L药液(含回神颗粒0.27 g)灌胃一次,此后再分别于每日早晚各灌胃一次,每次2 m L药液。分别于创伤后1 d、3d、7 d对正常组、假手术组、模型7 d组和治疗7 d组进行NSS量表评分;分别于创伤后1 d、3 d对模型3 d组和治疗3 d组进行NSS评分。分别于造模前和取材前称取每只动物的体重。观察NSS评分和体重的变化。结果:与正常组和假手术组比较,模型3 d组、模型7 d组、治疗3 d组、治疗7 d组各时间点NSS评分明显降低(P<0.01)。但与模型3 d组和模型7 d组的NSS评分相比较,治疗3 d组和治疗7 d组的NSS评分明显提高,具有显著性差异(P<0.01)。与正常组体重比较,模型组动物体重下降,具有明显差异(P<0.01);治疗组动物体重无明显下降(P>0.05)。比较每组动物造模前和取材前体重,发现模型组体重下降,具有明显差异(P<0.01);治疗组体重有所增加,具有明显差异(P<0.01)。结论:回神颗粒能明显改善TBI大鼠NSS评分,改善神经功能;促进大鼠的生长发育,减轻脑损伤。

Objective： Though the change of NSS and weight,this research deals with the mechanism of Huishen Granule alleviating moderate traumatic brain injury（ TBI） model rats. Method： NSS was used to evaluate normal group,sham group,model 7 day group,experimental 7 day group after TBI 1 day,TBI 3 days,TBI 7 days. NSS was used to evaluate model 3 day group,experimental 3 day group after TBI 1 day,TBI 3 days. Weighed every rats at the time of establishing model and killing rats and observed the change of NSS and weight. Results： Compared with normal group and sham group,the NSS of model 3 day group,model 7 day group,experimental 3 day group and experimental 7 day group declined obviously（ P〈0. 01）. But compared with model 3 day group and model 7 day group,the NSS of experimental 3 day group and experimental 7 day group increased significantly（ P〈0. 01）. Compared with the killed rats＇ weights of the model 3day group and model 7 day group,both the experimental 3 day group and experimental 7 day group increased significantly（ P〈0. 01）. Conclusions： Huishen Granule can increase the grade of NSS,promote upgrouth and alleviate TBI.