不同处理方法及不同部位的金花葵红外光谱研究

Infrared Spectroscopic Study of Hibiscus manihot. L with Different Methods and Different Parts

目的:对中药金花葵Hibiscus manihot.L的主要药用部位进行红外光谱及二阶导数图研究。方法:采用溴化钾压片法和傅里叶变换红外光谱法对金花葵的干燥花蕾、种子及果壳进行红外光谱及二阶导数谱进行研究。结果:不同处理方法的花蕾和种子的光谱有一定差异,花蕾和果壳的红外谱较为接近,主要在峰强度上存在不同。花蕾及果壳样品主要在3395、3026、2994、1708、1538、1345、1288、1205 cm-1等位置存在吸收峰,主要成分为黄酮、多糖、酯类等,种子特异性地含有脂肪烃(1720 cm-1)。结论:金花葵果壳的红外光谱表明其有相近于花蕾的化学成分,开发前景广阔,不同处理方法的样品具有差异性,该检测方法简便快捷,可用作金花葵的质量控制或样品甄别。可用于其它部位的研究,进一步加大资源利用率。

Objective： To research the main medicinal parts of TCM Hibiscus manihot. L via FTIR and the second order derivative spectra research. Method： FTIR and the second order derivative spectroscopy studies were carried on by using potassium bromide tableting and Fourier transform infrared spectroscopy of Hibiscus manihot. L＇s dry buds,seed and shell of were performed. Results： Flowers and seeds processed by different methods had differences to some extent. The infrared spectrum of buds was close to shells and those two differentiate in the peak intensity. Flower buds and fruit samples mainly in 3395,3026,2994,1708,1538,1345,1288,1205 cm- 1had absorption peaks and the main ingredients were flavonoids,polysaccharides,esters. Seeds specifically contained aliphatic hydrocarbons（ 1720 cm- 1）. Conclusion： FTIR spectra of Hibiscus manihot. L＇s shell shows that it is similar to the chemical constituents in flower buds. In terms of the development,there is a huge potential. As the methods adopted are different,the results will not be the same. However,the detection method employed in this study is efficient and convenient,which can be used as Hibiscus manihot. L＇s quality control or sample screening. Hibiscus manihot. L sample can be further made use of to study other parts of them.