大孔吸附树脂对草鱼蛋白水解液脱盐的作用

Desalination of Grass Carp Protein Hydrolysate Using Macroporous Adsorption Resin

为了脱除草鱼蛋白水解液中的盐,本研究筛选了4种型号大孔吸附树脂DA201-C、DA201-M、SQT-67和D002,对水解液中草鱼多肽的吸附能力进行测定,并进行静态吸附和解吸实验。结果表明:大孔吸附树脂DA201-C对草鱼多肽的吸附效果较好,其吸附量随草鱼多肽质量浓度的增加而增加;体积分数75%的乙醇对大孔吸附树脂DA201-C的洗脱效果最佳;DA201-C大孔吸附树脂对水解液脱盐的最佳条件为上样流速0.5 BV/h,上样质量浓度为30 mg/m L,水清洗速率为2 BV/h,洗脱采用体积分数75%的乙醇以2 BV/h的流速洗脱,在此条件下大孔吸附树脂DA201-C对水解液的脱盐率达97.17%。

In order to remove salt from grass carp protein hydrolysate, four macroporous adsorption resins including DA201-C, DA201-M, SQT-67 and D002 were selected for the highest adsorption capacity for grass carp peptides through static adsorption and desorption experiments. The results showed that DA201-C had the best adsorption efficiency for grass carp peptides. Its adsorption quantity increased with increasing concentration of grass carp peptides. Seventy-five percent ethanol revealed the best elution efficiency for DA201-C. The optimal conditions for the desalination of grass carp peptides with DA201-C were determined as follows： the sample at 30 mg/m L was passed through the column at a flow rate 0.5 BV/h, and then the column was washed with at a flow rate of 2 BV/h and eluted with 75% ethanol at 2 BV/h. Under these conditions, the desalination efficiency of grass carp peptides was 97.17%.