葛仙米藻胆蛋白提高小鼠免疫功能的研究(英文)

Nostoc sphaeroides Phycobiliprotein Enhances Immune Functions in Mice

研究葛仙米藻胆蛋白对昆明小鼠的免疫增强活性。分别以100、300、500 mg/(kg·d)的葛仙米藻胆蛋白溶液剂量对小鼠连续灌胃30 d。然后分别对腹腔巨噬细胞吞噬率、自然杀伤(natural killer,NK)细胞活性,T淋巴细胞增殖、细胞因子、抗体、迟发型超敏反应(delayed type hypersensitivity,DTH)等免疫参数进行测定。结果表明:葛仙米藻胆蛋白可以提高腹腔巨噬细胞吞噬率、NK细胞活性、T淋巴系统增殖以及血清中白细胞介素(interleukin-2,IL-2)和IL-4细胞因子水平。同时葛仙米藻胆蛋白处理组的小鼠DTH显著高于对照组,表明葛仙米藻胆蛋白是一种潜在的免疫增强剂。

Phycobiliprotein is a light-harvesting pigment in red and blue-green algae. It is widely used in the pharmaceutical industry due to its antioxidant activity and anticancer activity. In Nostoc sphaeroides Kützing, phycocyanin and phycoerythrin are major varieties of phycobiliprotein. However, the effect of Nostoc sphaeroides Küting phycobiliprotein（NSKP） on immune functions remains unclear. In the present study, the immunoenhancing effect of NSKP in Kunming mice was explored. The mice were administered with NSKP at dosages of 100, 300 and 500 mg/（kg·d）（body mass） by gavage once a day. After administration for 30 consecutive days, the immune parameters including the phagocytosis of peritoneal macrophages, natural killer cell activity, the proliferation of T lymphocytes, cytokines levels, antibody production, hemolytic complement activity and delayed-type hypersensitivity were measured. The results showed that NSKP promoted the phagocytosis of peritoneal macrophages, the function of natural killer cells, and the proliferation of T lymphocytes, as well as increased the levels of interleukin（IL）-2 and IL-4 and serum hemolysin. Meanwhile, the response of delayed-type hypersensitivity in NSKP group was also dramatically higher than that in the control group. Therefore, NSKP is a potential immunoenhancing agent.