脆江蓠多糖体内抗肿瘤活性及其作用机制

Antitumor Activity in Vivo and Mechanism of Action of Gracilaria chouae Polysaccharides

目的:研究脆江蓠多糖(Gracilaria chouae polysaccharides,GLP)的体内抗肿瘤活性。方法:以GLP为受试物,考察其对S180肉瘤荷瘤小鼠的抗肿瘤活性,同时通过测定小鼠机体免疫系统各项生化指标,初步探讨GLP的抗肿瘤作用机制。结果:GLP对S180荷瘤小鼠具有较强的抑瘤活性,当小鼠的GLP腹腔注射剂量为100 mg/(kg·d)(以体质量计)时,抑瘤率达到34.75%,高于中剂量灌胃组(200 mg/(kg·d))的抑瘤率(31.35%)。同时,S180荷瘤小鼠免疫器官指数极显著增大(P<0.01),GLP还激活了S180荷瘤小鼠淋巴细胞的增殖分化,S180荷瘤小鼠白细胞介素-2(interleukin-2,IL-2)、干扰素-γ(interferon-γ,IFN-γ)水平极显著上升(P<0.01),IL-10水平极显著下降(P<0.01),GLP对免疫系统显示出较好的保护作用。结论:GLP具有较强的抗肿瘤活性,且腹腔注射给药效果优于灌胃给药。GLP对小鼠机体免疫调节作用可能是其抗肿瘤作用的主要机制之一。

Objective： To investigate the antitumor activity of polysaccharides from Gracilaria chouae（GLP） in vivo. Methods： S180 sarcoma mouse model was established and used for the determination of antitumor activity of GLP in vivo. In addition, the underlying mechanism was explored by measuring biochemical parameters of immune system in the mice. Results： GLP definitely had inhibitory effect on S180 sarcoma in vivo. An inhibition rate of 34.75% was obtained with intraperitoneal injection at a dose of 100 mg/（kg·d）, higher than that（31.35%） of the medium-dose intragastric administration group（200 mg/（kg·d））. Immune organ indices of tumor-bearing mice significantly increased（P 〈 0.01）. Additionally, the proliferation and the differentiation of lymphocytes was activated by GLP administration. The levels of IL-2 and IFN-γ significantly increase（P 〈 0.01）, while the level of IL-10 significantly decreased（P 〈 0.01）. GLP showed good protection on the immune system. Conclusion： GLP had strong anti-tumor activity and was more effective when given by intraperitoneal injection than by intragastric administration. The anti-tumor effect of GLP was mainly achieved by improving immune function in mice.