老年人甲状腺乳头状癌组织中斯钙素1表达及其临床意义

Expression of stanniocaicin 1 in papillary thyroid carcinoma and its clinical significances in gerontal patients

目的探讨斯钙素1（stanniocaIcin1，STC1）蛋白在老年人甲状腺乳头状癌中的表达及其与临床病理特征的关系，分析老年患者与非老年患者斯钙素表达的差异。方法采用免疫组织化学二步法检测老年患者甲状腺乳头状癌、结节性甲状腺肿和正常甲状腺组织以及非老年患者甲状腺乳头状癌中STC1的表达水平，应用蛋白免疫印迹（Westernblot）检测不同甲状腺组织中STC1蛋白的表达。结果STC1在老年人甲状腺乳头状癌组的表达（60．9%），显著高于结节性甲状腺肿组（30．0％，P〈0．05）和正常甲状腺组（15．0％，P〈0．01）。甲状腺乳头状癌组织中STC1蛋白的相对表达量为0．647±0．076，也显著高于结节性甲状腺肿组（0．280±0．039，F=9．965，P〈0．01）和正常甲状腺组（0．248±0．065，F=1．143，P〈0．01）。甲状腺乳头状癌中STC1蛋白表达与患者的年龄、性别、肿瘤最大径和部位无关（P〉0．05），与肿瘤淋巴结转移以及临床分期相关（P〈0．05）。老年患者与非老年患者甲状腺乳头状癌组织中STC1表达比较，差异无统计学意义（F=0．007，P=0．934）。结论STC1蛋白与甲状腺乳头状癌发生发展相关，可能对甲状腺良恶性肿瘤的鉴别诊断、预后有一定参考意义。

Objective To investigate the protein expression of stanniocalcin 1 （STC1） in papillary thyroid carcinoma （PTC） in gerontal patients versus non-gerontal subjects, and its relationship with clinicopathological features. Methods The protein expression levels of STC1 in PTC, nodular goiter and normal thyroid tissues in the gerontal patients versus non-gerontal subjects were detected by immunohistochemistry. The protein expressions of STC1 were detected by Western blotting. Results The protein expression of STC1 in gerontal patients was higher in PTC than in nodular goiter and normal thyroid tissues （60.9% vs. 30.0%, 15.0%, P〈0.05 or 0.01）. The relative expression of STC1 protein in gerontal patients was higher in PTC than in nodular goiter and normal thyroid tissues [（0. 647 ± 0. 076） vs. （0. 280± 0. 039）, （0. 248± 0. 065）, F= 9. 965 and 1. 143, both P〈0.01]. STC1 protein expression had no correlations with age, gender, tumor diameter and tumor position in patients （P〈0. 05）, while it was associated with tumor lymph node metastasis and clinical stage （P〈0. 05）. There was no significant difference in above indexes between the elderly and non-elderly patients with papillary thyroid carcinoma （F=0. 007, P=0. 934）. Conclusions STC1 protein may be associated with the development of papillary thyroid carcinoma, and it may has a some reference value in differentiating benign from malignant thyroid neoplasms and in predicting the prognosis of thyroid carcinomas.