不同栽培条件下辣椒果实辣椒素含量的分析与QTL定位

Analysis and QTLs Identification of Pepper Fruit Capsaicin Content under Different Cultivation Conditions

利用辣味辣椒Pemnnial（Capsicum annuum）和无辣味辣椒83—58种内重组自交系群体构建的辣椒种内遗传图谱，以温室和露地栽培条件下无辣味辣椒77013A与重组自交系群体各株系杂交F1果实的辣椒素和二氢辣椒素含量作为表型性状进行分析。结果表明，温室和露地栽培条件下辣椒素和二氢辣椒素的含量和比值差异明显。对露地和温室栽培条件下辣椒果实辣椒素和二氢辣椒素含量、总和及其比值进行了QTI．定位，共获得16个关于辣椒素和二氢辣椒素含量的QTL位点，分布在辣椒第2、4、12号染色体上，2号染色体上同时控制辣椒素、二氢辣椒素、二者比值和辣椒素总量的主效QTL位点cap21、dhp2．J、C／D2．1和（C＋D）2．1，均在露地和温室被检测到，其LOD值大于5．0，贡献率为20．2％-76．6％，侧翼标记均为BD76366和Punl，12号染色体上调控辣椒素总量的微效QTL也在温室和露地同时被检测到，其他QTL位点未在两种环境下同时定位到。

In lhe present study, we eonstructed an intraspeeifie genetic map by using a recombinant inbred lines erossed by pungent pepper ‘Perennial＇ （Capsicum annuum）and non-pnngent pepper ‘83-58＇, and analyzed capsaiein and dihydroeapsaiein contents investigated on a test cross progeny by non-pungent pepper ‘77013A ＇ and RILs （ ‘83-58＇ בPerennial＇ ） both in greenhouse and open fields. And the differences in eapsaiein content, dihydrocapsaiein conlent, tolal eapsaieinoids and eapsaiein/dihydroeapsaiein ratio between greenhouse and open fields were obvious. We did QTL mapping on them and found 16 QTLs located in chromosome 2, 4 and 12. The major QTL cap2.1, dkp2.1, C/D2.1 and （C＋D） 2.1 on chromosome 2 that controlling eapsaiein eontenl, dihydroeapsaiein content, tolal eapsaieinoids content and eapsaiein/dihydroeapsaiein ratio, was deteeled ill both greenhouse and open fields. The LOD was above 5.0, aeeounting for 20.2%-76.6% of the phenolypie variation. The interval markers were BD76366 and Punl. We also found minor QTLs in P12 that regulating lotal eapsaieinoids content in 2 environments. Other minor QTLs were not detected at the same time in these 2 environments.