乏氧、去血清抑制KAI1的生长抑制功能

Hypoxia and serum deprivation protected MiaPaCa-2 cells from KAI1-induccd proliferation inhibition

目的观察乏氧和缺血是否影响KAI1的生长抑制作用。方法应用无KAI1表达的人胰腺癌细胞MiaPaCa2，通过感染带有KAI1目的基因的复制缺陷型腺病毒Ad5-KAI1，使细胞表达KAII蛋白，通过乏氧和去血清培养细胞模拟实体瘤内缺血、缺氧的微环境，根据培养条件不同，将人胰腺癌细胞株MiaPaCa-2分为4组：对照组（正常培养）、乏氧组、去血清组、乏氧去血清组。用CCK8和Annexin V～FITC／PI实验检测细胞增殖和凋亡的变化。结果乏氧和去血清培养细胞明显拈抗KAI1的增殖抑制和诱导凋亡作用，进而促进细胞的生存。结论乏氧和去血清拮抗KAI1的生长抑制作用。

Objective KAI1 closely correlates with pancreatic cancer metastasis.There might be some factors that protect the cells from a proliferation inhibition by KAI1 in the solid tumors＇ microenvironment.Hypoxia and ischemia are the main characteristics of the microenvironment within solid tumors.Whether they affect the KAI1 inhibitory effects on cell proliferation is still unclear. Methods MiaPaCa-2 human pancreatic cancer cells do not express KAI1 protein.However, after being infected with Ad5-KAIl,they expressed KAIl protein.cultured them under hypoxic and serum-free conditions to simulate the solid tumor hypoxic-ischemic microenvironment.The cells were divided into the control,hypoxic, serumfree,and hypoxic with serum-free groups.The proliferation and apoptosis were observed by CCK8 and Annexin V-FITC/PI,respectively.We, hen observed whether the hypoxic and serum-free conditions could change the effect of KAI1 on cell survival. Results Hypoxia and serum-free media effectively reduced the apoptosis and proliferation inhibition caused by KAI1 and was beneficial to the cell survival. Conclusions Serum free media and hypoxia protected the MiaPaCa-2 cells from a KAIl-induced apoptosis and proliferation inhibition.