HPLC法测定野蔷薇中齐墩果酸和熊果酸的含量

Determination of oleanolic acid and ursolic acid in Rosa Multiflora Thunb. by HPLC

目的：采用HPLC法测定野蔷薇不同部位（根、茎、果实）中齐墩果酸和熊果酸的含量,比较不同产地野蔷薇果实中齐墩果酸和熊果酸含量的差异。方法：采用Zorbax ODS色谱柱（4.6 mm×250 mm×5μm）,以甲醇-水（88∶12）为流动相,流速为1.0 mL/min,检测波长为210 nm。结果：齐墩果酸和熊果酸在20 min内较好分离,其质量浓度分别为0.02468-0.2468 mg/mL和0.02523-0.2523 mg/mL时与峰面积线性关系良好,相关系数分别为0.9994和0.9996;平均回收率为分别为99.96%（RSD为0.74%）和100.03%（RSD为0.95%）。结论：该方法操作简便、结果准确、重现性好,可同时测定野蔷薇中齐墩果酸和熊果酸的含量,均在果实中含量最高;库车县、阿图什市的野蔷薇果实中齐墩果酸和熊果酸的含量最多,可作为最适开发利用的固定来源。

Objective：The content of oleanolic acid（OA） and ursolic acid（UA） in different arts（roots,stems,fruits）of Rosa Multiflora Thunb. have been compared,and taken analysis on content of OA and UA in Rosa Multiflora Thunb. fruit from different origin by HPLC. Methods ：Zorbax ODS chromatography column（4.6 mm×250 mm×5 μm）,methanol-water（88∶12） was used as mobile phase,diode array detector at 210 nm wavelength had been used for detection. Results：The OA and UA was better separated within 20 minutes,it had good linear relationship between peak area mass concentration between 0.02468-0.2468 mg/mL and 0.02523-0.2523 mg/mL with the correlation coefficient of 0.9994 and 0.9996 respectively. The recovery rates were 99.96%（RSD 0.74%）and 100.03%（RSD 0.95%）. Conclusion：The established method was simple,quick,accurate,and had better reproducibility for the determination of OA and UA in Rosa Multiflora Thunb. at the same time by HPLC. The content of OA and UA in fruits reached the highest value. The Rosa Multiflora Thunb. from Kuqa and Atux had the highest content of OA and UA,so that could be used as the origin for the research of Rosa Multiflora Thunb.