摘要
采用分子筛层析法从人工培养蛹虫草子实体中分离纯化得到一种多糖W-CBP80Ⅰ,进而分别采用凝胶渗透色谱(GPC)、气-质联用(GC-MS)、红外光谱(IR)、核磁共振(~1H NMR和^(13)C NMR)研究其理化性质和结构特征。结果表明:W-CBP80Ⅰ含有α-糖苷键,并主要由甘露糖、半乳糖和葡萄糖组成。它主要成分为低分子量多糖,分子量为8.93×10~3。此外,W-CBP80Ⅰ具有体外清除DPPH,羟基和超氧阴离子自由基活性。
A polysaccharide W-CBP80 Ⅰ was isolated from the fruiting bodies of cultured Cordyceps militaris and characterized using GPC,GC-MS,IR,^1H NMR and ^13C NMR. The results indicated that it contained α-type glycosidic linkages and was mainly composed of α-mannose,α-galactose and α-glucose. Its main component was low molecular weight polysaccharide with Mwof 8.93×10^3. In addition,W-CBP80Ⅰ exhibited scavenging capacities against DPPH,hydroxyl and superoxide radicals in the antioxidant assay in vitro.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第6期155-159,共5页
Science and Technology of Food Industry
关键词
蛹虫草
多糖
结构特征
抗氧化
Cordyceps militaris
polysaccharide
structure characterization
antioxidation
