超声波辅助提取水酶法豆渣中植酸的工艺优化

Ultrasonic assisted in the extraction of phytic acid from soybean residue produced by enzymatic aqueous processing

以水酶法提油后的副产物豆渣为原料,探索超声波辅助技术提取植酸的工艺条件,并与振荡浸提法对比。首先选取超声功率、超声温度、超声时间进行单因素实验,通过正交实验选出最佳的超声参数。然后在最优超声参数的基础上,以植酸得率为响应值,利用Box-Behnken中心组合设计原理和响应面分析法,确定提取植酸的最佳条件。结果表明,超声提取植酸的条件为:超声温度50℃,超声功率500 W,超声时间20 min;醋酸浓度4.1%、料液比1∶17 g/m L、提取温度64℃、提取时间33 min。在以上条件下,平均植酸得率为1.23%,与振荡提取法相比,提高41.3%。

Soybean residue produced by enzymatic aqueous processing of soybean oil was used as the raw material to prepare phytic acid through the steps of ultrasonic-assisted extraction and compared with shaking extraction. Optimal ultrasonic conditions were determined by single factor and orthogonal tests,selecting ultrasonic power,ultrasonic temperature and ultrasonic time. On the basis of ultrasonic treatment,optimal extraction conditions of phytic acid were studied by using Box-Behnken central composite design and response surface analysis theory. Results showed that the optimal ultrasonic conditions were ultrasonic temperature50 ℃,ultrasonic power 500 W and ultrasonic time 20 min. The optimal extraction conditions were acetic acid concentration 4.1%,ratio of solid to liquid 1∶17 g/mL,extraction temperature 64 ℃,extraction time 33 min. Under these conditions,the average phytic acid yield was 1.23%,which was higher than that of shaking extraction by 41.3%.