显齿蛇葡萄叶总黄酮对原发性高血压大鼠的降血压研究

Effect of the flavonoid fraction of Ampelopsis grossedentata on spontaneously hypertensive rats

为了探讨显齿蛇葡萄叶总黄酮对原发性高血压大鼠的影响,采用超声波辅助提取,D-101大孔树脂纯化制备显齿蛇葡萄叶总黄酮,将SHR大鼠40只随机分成正常组、模型组、显齿蛇葡萄叶黄酮组(100、200和400 mg/kg)和依那普利组(5 mg/kg),显齿蛇葡萄叶黄酮组和依那普利组按照体重分别给予对应药物灌胃10周,分别在10周和12周测定血压及血清中各项指标。同时利用显齿蛇葡萄叶黄酮不同剂量喂养正常大鼠6个月,测定血压及血清各项指标。结果显示,显齿蛇葡萄叶黄酮治疗原发性高血压大鼠10周后能明显降低血压,另外也降低血清中血浆肾素、血管紧张素Ⅱ、内皮缩血管肽和丙二醛的含量和提高一氧化氮、超氧化物歧化酶、过氧化氢酶、谷胱甘肽的含量,对正常Wistar大鼠喂养后发现对血压基本没有影响。提示显齿蛇葡萄叶黄酮对原发性高血压大鼠具有明显的降血压效果,潜在的机理可能与肾素-血管紧张素的-醛固酮系统(RAAS)调制和抗氧化系统有关,另外显齿蛇葡萄叶黄酮对正常大鼠的血压没有影响,表明显齿蛇葡萄叶黄酮可用来作为天然药物或功能食品来预防高血压。

The present study aimed to assess the effect of the total flavoniods Ampelopsis grossedentata on（TFA） spontaneously hypertensive rats and normotensive rats,TFA was extracted by ultrasonic method and purified by D-101 macroporous resin. The spontaneously hypertensive rats in experimental group were intragastrically administrated with TFA at 100,200 and 400 mg/kg once a day for consecutive 10 weeks. Rats were given intragastrically with 5 mg/kg enalapril as controls. At end of 10 week,some rats were randomly selected for blood pressure determination and were anesthetized for serum preparation. After keeping normally for 2 weeks,the remaining rats were subjected to blood pressure measurement and serum isolation. The concentration of PRA,ANG Ⅱ,ET,NO,SOD,CAT,GSH,MDA were evaluated. After the healthy SD rats were treated with TFA for 6 months,the blood pressure,the concentration of Na＋,K＋,Cl-and Na＋/K＋were measured.TFA significantly reduced the blood pressure,the concentration of PRA,ANG II,ET and MDA in serum and increase the level of NO,SOD,CAT,GSH in primary hypertension rats for 10-week treatment. There was no significant changes in blood pressure of control rats after TFA treatment. TFA significantly reduced the blood pressure in primary hypertension rats. The potential mechanism may be related with the antioxidant system regulation by RAAS. No significant effect was observed on healthy rats. All these suggested that ampelopsis grossedentata showed significant effect on preventing hypertension as natural medicine and natural function food.