激素剂量对激素性股骨头坏死模型建立的影响及系统评价

The Influence of Glucocorticoids Doses of Steroid-induced Necrosis of Femoral Head(SANFH) Model and System Evaluation

[目的]确定建立激素性股骨头坏死(steroid-induced necrosis of femoral head,SANFH)动物模型的最佳激素剂量。[方法]采用激素联合内毒素诱导造模,SD大鼠随机分为空白对照组、甲基强的松龙10 mg·kg-1组、甲基强的松龙20mg·kg-1组、甲基强的松龙40mg·kg-1组、甲基强的松龙80mg·kg-1组。除空白对照组外,其余各组尾静脉注射大肠杆菌脂多糖1次/d,注射2d,剂量为40μg·kg-1,末次注射24h后,臀肌注射相应剂量甲基强的松龙(10、20、40、80mg·kg-1)1次/d,注射3d,建立SANFH模型;空白对照组臀肌注射等体积生理盐水。观察造模后大鼠体重、骨密度、骨组织形态学、骨组织病理学、骨生物力学、骨钙磷等指标的变化。[结果]当注射激素剂量为40mg·kg-1时,大鼠体重较空白对照组增长(P<0.05,P<0.01),大鼠股骨密度、最大弯曲载荷、杨氏模量均显著降低(P<0.05,P<0.01)。造模8周以后,甲基强的松龙40mg·kg-1组骨钙、骨磷含量降低(P<0.05)。[结论]建立SANFH动物模型最佳甲基强的松龙激素剂量为40mg·kg-1。

[Objective]To determine the best glucocorticoids dose to establish animal model of SANFH. [Methods]Use LPS and glucocorticoids to establish the SANFH model of rats. Screen the dose of glucocorticoids. The dose of glucocorticoids（10, 20, 40,80mg·kg-1）, except the blank group, the rest of the group intravenous injection LPS for 2 days, blank control group with gluteal muscle injection of saline solution, to establish the animal model of SANFH. To observe the change of body weight, bone histomorphometry, bone tissue pathology index after modeling. [Results]When the glucocorticoids at the dose of 40mg·kg-1, the body weight of rats increased.The density of rat femur, the maximum bending load and displacement and Young＇s modulus all significantly reduced. After modeling for 8 weeks, the bone calcium,pbosphorus content of methylprednisolone 40mg·kg-1 group was lower. [Conclusion] The best glucocorticoids dose to establish animal model of SANFH is 40mg·kg-1.