胃肠道巨细胞病毒感染性疾病的组织学检测及应用

Histologic detection of cytomegalovirus and its application in gastrointestinal cytomegalovirus infection

目的探讨胃肠道巨细胞病毒（CMV）感染性疾病的临床病理特点及组织学检测的方法和效果。 方法收集2009年7月至2015年7月临床疑诊消化道CMV感染的患者35例，组织学标本41份，HE切片观察组织学形态，并行CMV免疫组织化学及原位杂交检测CMV感染细胞。组织学检测结果与患者血标本CMV DNA、pp65抗原等检测结果比对。 结果41份标本中23份检测到CMV感染细胞，32份组织学检测前后1周内有血DNA检测，结果与组织学检测比较，血DNA检测的敏感性73.7%，特异性69.2%。 结论胃肠道组织学检测CMV在临床实践中具有重要应用价值，HE结合免疫组织化学和/或原位杂交检测是可靠的检测方法。

ObjectiveTo investigate the clinical and pathologic features of gastrointestinal cytomegalovirus （CMV） disease, and the histological detection of CMV. MethodsForty-one gastrointestinal tissues were obtained from 35 patients suspected for gastrointestinal CMV disease. The pathologic changes of these specimens were evaluated by immunohistochemistry and in situ hybridization for CMV, and these were compared to serologic detection methods. ResultsCMV was detected in 23/41 tissues. There were 32 serologic CMV DNA tests at the time of biopsies or operations. Compared with histological detection, the sensitivity and specificity of serologic CMV DNA tests were 73.7% and 69.2% respectively. ConclusionsGastrointestinal histological detection of CMV has important clinical application. HE staining combined with immunohistochemistry and/or in situ hybridization detection is a reliable method to detect CMV.