摘要
Heat shock cognate protein 70 （Hsc70） is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quan- titative polymerase chain reaction and Western-blot techniques to analyze the transcrip- tional and translational expression profiles of AIHSC70 under extreme temperature （4℃ and 40℃） or 4 pesticide stresses in Apolygus lucorum. The results showed that the expres- sion of AIHSC70 were significantly induced by cyhalothrin or extremely high temperature （40℃） in both transcriptional and translational levels （P 〈 0.05）, while the transcriptional and translational level of AIHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature （4℃） （P 〈 0.05）. Moreover, afterApolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AIHSC70 was only up-regulated significantly at the transcriptional level （P 〈 0.05）, although obviously up-regulated at the translational level of AIHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature （40℃）. In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages （P 〈 0.01）, indicating temperature was an important factor to affect the relative expression of Alhsc70.
Heat shock cognate protein 70 （Hsc70） is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quan- titative polymerase chain reaction and Western-blot techniques to analyze the transcrip- tional and translational expression profiles of AIHSC70 under extreme temperature （4℃ and 40℃） or 4 pesticide stresses in Apolygus lucorum. The results showed that the expres- sion of AIHSC70 were significantly induced by cyhalothrin or extremely high temperature （40℃） in both transcriptional and translational levels （P 〈 0.05）, while the transcriptional and translational level of AIHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature （4℃） （P 〈 0.05）. Moreover, afterApolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AIHSC70 was only up-regulated significantly at the transcriptional level （P 〈 0.05）, although obviously up-regulated at the translational level of AIHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature （40℃）. In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages （P 〈 0.01）, indicating temperature was an important factor to affect the relative expression of Alhsc70.
基金
Acknowledgments This work was supported by the Natural Science Foun- dation of Jiangsu Province （BK20130717）, the Jiangsu Agriculture Science and Technology Innovation Fund （JASTIF）, CX（14）5020, the National Natural Science Foundation of China （31401753）, the Open Fund in State Key Laboratory for Biology of Plant Diseases and Insect Pests （SKL2012OP03）, the Special Fund for Agro-scientific Research in the Public Interest of China （201103012-04）. We thank Professor Zhong-Yan Gu for providing the pesticide AI for this study.
