化痰法为指导的白芥子相关药组对肝纤维化大鼠VEGF、bFGF表达的影响

Effects of Semen Sinapis Albae Related Medicine Groups on the Expression of VEGF and b FGF in Rats with Hepatic Fibrosis under the Guidance of Resolving Phlegm

目的:对白芥子及其药组对实验性肝纤维化大鼠VEGF、b FGF、CoⅣ、LN的影响进行研究。方法:60只雄性SD大鼠随机分为空白对照组、模型组、阳性对照组、白芥子组、白芥子+丹参组、白芥子+黄芪组。除空白对照组外,其余各组均采用大鼠背部皮下注射50%CCl40.1 m L·100 g-1,2次·周-1,连续造模12周。阳性对照组、白芥子组、白芥子+丹参组、白芥子+黄芪组于造模的第7周开始给药,阳性对照组给予秋水仙碱0.4 mg·kg-1,白芥子组、白芥子+丹参组、白芥子+黄芪组的剂量分别为1.0 g·kg-1,共7周。采用放射免疫分析法检测肝组织匀浆CoⅣ、LN,免疫组织化学法检测各组大鼠肝组织VEGF、b FGF蛋白的表达。结果:模型组与正常组比较:CoⅣ、LN显著增高(P<0.01),VEGF、b FGF蛋白的表达显著增高(P<0.05);白芥子组、白芥子+丹参组、白芥子+黄芪组与模型组比较:CoⅣ、LN显著减低(P<0.05),VEGF、b FGF蛋白的表达显著减低(P<0.05)。结论:以化痰理论为指导的白芥子及其药物组合对肝纤维化大鼠有一定的防治作用,其机制可能与影响VEGF、b FGF蛋白的表达有关。

Objective： To study the effects of semen sinapis albae related medicine groups on VEGF,b FGF,CoⅣ and LN in rats with hepatic fibrosis. Methods： 60 male SD rats were randomly divided into blank control group,model group,positive control group,semen sinapis albae group,semen sinapis albae and salvia miltiorrhiza group,semen sinapis albae and astragalus membranaceus group. Rats of all groups except the control group were modeled by hypodermic injection of0. 1 m L·100 g- 150% CCl4 twice per week for 12 weeks continuously. The positive control group,semen sinapis albae group,semen sinapis albae and salvia miltiorrhiza group,semen sinapis albae and astragalus membranaceus group were given medicine at the beginning of the seventh week; the positive control group was given colchicine 0. 4 mg·kg- 1; semen sinapis albaegroup,semen sinapis albae and salvia miltiorrhiza group,semen sinapis albae and astragalus membranaceus group were given corresponding medicines 1. 0 g·kg- 1,7 weeks in all. Radioimmunoassay method was adopted to detect the level of CoⅣ and LN in the liver homogenate. Immunohistochemical method was employed to detect the expression of VEGF and b FGF in liver tissues of rats in each group. Results： Compared with the normal group,the level of CoⅣ and LN in the model group were significantly increased（ P〈0. 01）,and the expression of VEGF and b FGF were significantly increased（ P〈0. 05）. Compared with the model group,the level of CoⅣand LN in the semen sinapis albae group,semen sinapis albae and salvia miltiorrhiza group,semen sinapis albae and astragalus membranaceus group were significantly reduced（ P〈0. 05）,and the expression of VEGF and b FGF were significantly reduced（ P〈0. 05）. Conclusion： The semen sinapis albae related medicine groups under the guidance of resolving phlegm have a certain preventive and therapeutic effect on hepatic fibrosis rats. The mechanism may be related to the expression of VEGF and b FGF.