渗透树脂与牙本质直接粘接的体外研究

Adhesive performance of infiltrating resin bonded on dentin in vitro

目的 研究渗透树脂与牙本质直接粘接的微观形态及微拉伸强度,为临床应用提供参考.方法 选取20颗新鲜离体牙,磨除表面釉质和部分牙本质,将渗透树脂（ICON infiltrate,DMG,德国）与牙本质和复合树脂直接粘接制备树脂-牙块（渗透树脂组）,对照组粘接剂为自酸蚀粘接剂（Clearfil SE Bond adhesive,Kuraray,日本）,每组10个树脂-牙块.在粘接即刻和冷热循环（10000次）后,将树脂-牙块切割为片状或长方体,扫描电镜观察片状试件粘接界面的微观形态,微拉伸强度实验测定长方体试件的微拉伸强度,每组冷热循环前、后各10个片状试件和长方体试件.采用方差分析和LSD法进行统计学分析.结果 渗透树脂能渗入经前处理剂处理后的牙本质中,并形成长而密的树脂突.冷热循环前后微拉伸强度渗透树脂组分别为（35±10）和（35±9） MPa,两者差异无统计学意义（P＞0.05）;对照组分别为（38±8）和（24±7） MPa,冷热循环后微拉伸强度显著下降（P＜0.05）;冷热循环后,渗透树脂组微拉伸强度显著高于对照组（P＜0.05）.结论 渗透树脂能有效渗入经前处理剂处理后的牙本质,并获得类似自酸蚀牙本质粘接剂的粘接强度和抗老化性能.

Objective To evaluate the adhesive interface and micro-tensile bond strength（μTBS） of infiltrating resin directly bonded to normal dentin.Methods Twenty extracted human molars were collected and ground to expose fresh dentin surface.An infiltrating resin（ICON,DMG,Germany） was served as experimental group and Clearfil SE Bond adhesive（Kuraray,Japan） as control group.Following the application of primer（Kuraray,Japan）,the specimens were applied infiltrating resin or adhesive respectively and blocks of composite resin were built up.The adhesive interfaces were observed using scanning electron microscope（SEM） and the μTBS was measured by micro-tensile test before and after thermal cycling.Results The infiltrating resin could penetrate into micro-structure of dentin created by SE Bond primer.A layer of about 180 μm-long and dense resin tags was observed under SEM in infiltrating resin group.The μTBSs were （35± 10） MPa before and （35±9） MPa after thermal cycling respectively in infiltrating resin group,and the difference was not statistically significant（P〉0.05）.The μTBSs were （38±8） MPa before and （24±7） MPa after thermal cycling respectively in control group,and the difference was statistically significant （P〈0.05）.After thermal cycling,the μTBS of infiltrating resin group was significant higher than that of control group.Conclusions The infiltrating resin could penetrate into the micro-structure of dentin created by SE Bond primer.The bond strength and the bonding durability of infiltrating resin were similar to that of SE Bond adhesive.