中国地方黄牛PPARα exon7基因多态性与生长性状的关联研究

Polymorphism of the Exon 7 of PPARαGene and Correlation with Growth Traits in Chinese Indigenous Cattle

[目的]以期为我国地方黄牛生长性状分子育种提供理论依据。[方法]本研究以南阳牛、郏县红牛、鲁西牛和秦川牛4个品种共计424头中国地方黄牛为材料,以与牛脂肪代谢相关的基因PPARα为目标基因,利用生物信息学、PCR-RFLP结合DNA池测序等技术,对其第7外显子(PPARα_exon7)进行遗传特征分析,检测SNPs,并分析该序列的多态性与试验黄牛生长性状指标的相关性。[结果]发现在PPARα基因(AC_000162.1)exon7的164bp处发现了一处SNP(C/T),检测出CC和CT两种基因型。群体遗传分析显示,该位点在试验牛群体中均属于中度多态(0.25<PIC<0.5);关联分析结果显示,该位点在体斜长指标上,两种不同基因型的个体之间有显著的差异(P<0.05)。[结论]表明该位点有可能作为黄牛生长性状辅助选择的标记之一,应用于中国地方黄牛的分子遗传育种工作中。

【Objective】The aim of this study was to provide theoretical basis for the molecular breeding of local cattle growth traits in China.【Method】In order to better understand the effect of PPARαgene which related with bovine fat metabolism in 424 Chinese indigenous cattle samples,including Nanyang cattle（NY）,Jiaxian red cattle（JX）,Luxi cattle（LX）and Qinchuan cattle（QC）.A novel SNP of exon 7locus of the bovine PPARαgene was detected with bioinformatics,PCR-RFLP,and DNA sequencing to investigate the genetic variations of PPARα.In addition,an association study was carried out to determine the effect of the SNPs of PPARαgene in exon 7on the growth and meat quality traits.【Result】As a result,the SNP（C/T）was identified at 164 bp of PPARαgene in exon 7（AC_000162.1）,which contained two genotypes（CC and CT）.The genetic analysis showed that SNP（C/T）was midrange polymorphism（0.25PIC0.5）,and the association analysis of CC and CT genotypes with the growth and development traits indicated that individuals had a significant difference in body length（P 0.05）.【Conclusion】Therefore,this SNP（C/T）of PPARαgene in exon 7maybe one marker in selecting individuals with superior growth and meat quality traits,which could be used in molecule breeding for Chinese indigenous cattle.