HPLC-PAD同时测定玛咖中8种酰胺类化合物含量

Simultaneous determination of eight macamides in Lepidium meyenii Walp.by HPLC-PAD

为了建立同时测定玛咖中亚麻酰胺、亚油酰胺、十六烷酰胺、甲氧亚麻酰胺、甲氧亚油酰胺、甲氧十六烷酰胺、油酰胺和十八烷酰胺含量的HPLC-PAD方法,样品用正己烷超声波辅助提取,采用Waters Symmetry Shield色谱柱（C18,5μm,4.6 mm×250.0 mm）,以乙腈-水溶液为流动相进行梯度洗脱,检测波长为200 nm,外标法定量。结果显示,8种酰胺类化合物在0.05~10.00 mg/L具有良好的线性,相关系数≥0.999,检出限为0.08~0.22 mg/kg,定量限为0.26~0.76 mg/kg。20 mg/kg、50 mg/kg和100 mg/kg添加水平的加标样回收率为77.0%~117.0%,相对标准偏差为1.58%~5.12%。表明该方法操作简单,重复性和稳定性好,适合玛咖中8种酰胺类化合物同时检测和定量分析。

To establish a HPLC-PAD method to determine eight macamide components,N-benzyl-（ 9Z,12 Z,15Z）-octadecadienamide,N-benzyl-（ 9Z,12 Z,15Z）-octadecatrienamide,N-benzyl-（ 9Z,12Z）-octadecadienamide,N-benzyl-（ 9Z,12Z）-octadecadienamide,N-（ 3-methoxybenzyl）-hexadecanamide,N-（ 3-methoxybenzyl）-benzyloctadecanamide,Nbenzyl-（ 9Z）-octadecenamide,and N-benzyloctadecanamide in Lepidium meyenii Walp.,the sample was extracted by hexane with ultrasound assistance. The chromatographic separation was performed on Waters Symmetry Shield column（ C18,5 μm,4.6 mm×250. 0 mm） by using water solution-acetonitrile as the mobile phase. The photodiode array detector（ PAD） wavelength was set at 200 nm. The calibration curve was liner in the range of 0.05-10. 0 mg / L with the correlation coefficients more than 0. 999. The limit of detection was from 0. 08 to 0. 22 mg / kg and the limit of quantification was from 0. 26 to 0. 76 mg / kg for eight macamide components. The spiked recoveries of 20 mg / kg,50 mg / kg and 100 mg / kg ranged from 77. 0% to 117. 0%,with the relative standard deviations（ RSDs） being 1.58%- 5. 12%. The method showed good repeatability,accuracy and stability,and could be applied in the accurate quantification of eight macamide components in Lepidium meyenii Walp..