6株鹅细小病毒的分离及其VP3基因序列分析

Isolation of 6 Strains of Goose Parvovirus and Analysis of VP3 Gene

为了解吉林省不同地区小鹅瘟流行情况,从吉林省磐石、白城、九台、德惠、辽源与镇赉地区鹅养殖场采集疑似小鹅瘟雏鹅的肠管,处理后接种SPF鹅胚成功分离到6株病毒（暂命名为PSH、BCH、JLJT、DH、LY与ZL株）,经PCR鉴定为鹅细小病毒。VP3基因序列分析结果显示,分离到的6株鹅细小病毒VP3基因与近年来国内流行毒株同源性较高,核苷酸与氨基酸同源性分别为93.1%-99.6%与95.9%-99.4%。其中,BCH株、JLJT株与LY株属同一分支,与浙江和扬州等地区分离的鹅细小病毒相似性较高,达到99.4%-99.6%;ZL株、DH株与PSH株属同一分支,相似性较低。试验表明,吉林省流行的鹅细小病毒与我国南方地区流行毒株同源性较高,与其他地区流行毒株存在一定差异。

In order to evaluate the epodemic development of goose parvovirus（ GPV） in different district of Jilin province,intestines of suspected goose were collected from poultry farm in different regions including Panshi,Baicheng,Jiutai,Dehui,Liaoyuan and Zhenlai city of Jilin province,and 6 strains named respectively PSH,BCH,JLJT,DH,LY and ZL,were isolated with inoculating SPF goose embryos. The detection of PCR showed that all six samples were positive for goose parvovirus. The result of sequence analysis of VP3 gene showed that the 6 isolated strains shared high identities with the domestic popular strains in recent years,with 93. 1% ~ 99. 6% and 95. 9% ~99. 4% homology. Among them,BCH,JLJT and LY located in the same branch,all three strains had a high homology at 99. 4% ~ 99. 6% with isolayed strains from Zhejiang and Yangzhou; ZL,DH and PSH located in the same branch,but the homology was low. This assay indicate that the epidemic GPV strain in Jilin province possessed high identities with epidemic strain of the southern China,and different from strains in other areas.