酶解辅助提取陈皮中橙皮苷的工艺优化

Optimization of Enzyme-assisted Extraction Process for Hesperidin from Citrus reticulata

为了优化纤维素酶辅助提取陈皮中橙皮苷的工艺,在单因素试验基础上,选取酶解温度、酶解p H值、酶用量、酶解时间作为自变量,以橙皮苷收率为响应值,利用Box-Behnken中心组合设计原理和响应面分析法,研究各自变量及其相互作用对橙皮苷提取效果的影响,并优化该酶解辅助提取条件。优选得到橙皮苷的最佳酶解辅助提取工艺为:酶解温度50℃、酶解p H值5.0、酶用量8.9 mg/g、酶解时间47 min。在此最佳工艺条件下,纤维酶辅助提取橙皮苷的收率为5.24 mg/g,与理论预测值(5.26 mg/g)的相对误差仅为0.38%。优选的提取工艺简单、稳定,为工业化生产提供参考依据。

Hesperidin from Citrus reticulata was extracted by cellulase- assisted extraction process. On the basis of single factor experiments,enzymolysis temperature,p H value,cellulase dosage and enzymolysis time were chosen as independent variables and hesperidin yield as response value. Box- Behnken central composite design and response surface methodology were adopted to study the interaction of the receptive variables and their influence on the hesperidin yield,as well as the optimized extraction process. The optimum extraction condition were as follows： enzymolysis temperature 50 ℃,p H 5. 0,cellulase dosage 8. 9 mg / g,enzymolysis time 47 min.Under this condition,the hesperidin yield was up to 5. 24 mg / g,the relative error was merely 0. 38% compared to the predicted value（ 5. 26 mg / g）. The optimum extraction condition is simple and stable,and which serves as references for industrial large scale production.