逍遥丸对肝郁脾虚证非酒精性脂肪肝病大鼠TLR4表达的影响

Effect of xiaoyao pill on expression of TLR4 in rats with nonalcoholic fatty liver disease of liver depression and spleen deficiency syndrome

目的:观察逍遥丸对肝郁脾虚型非酒精性脂肪肝病大鼠肝脏TLR4表达的干预作用。方法:32只SPF级SD大鼠随机分为空白对照组、模型组、逍遥丸组、甘氨酸组每组8只。空白对照组给予基础饲料喂养;其余组均给予高糖高脂饲料及饮食不节、慢性束缚应激刺激共14周以复制模型;逍遥丸组、甘氨酸组在造模的基础上分别给予逍遥丸、甘氨酸灌胃,1次/d,连续30天后,腹主动脉取血,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、转氨酶(ALT)、5-羟色胺(5-HT)、去甲肾上腺素(NE)、D-木糖排泄率;HE染色观察肝脏形态变化;Western b Lotting检测TLR4的蛋白表达。结果:与空白组比:模型组血清TC、TG、ALT水平显著升高;HDL-C水平显著降低;5-HT、NE、D-木糖排泄率显著降低;肝细胞胞浆内出现脂肪空泡;肝脏TLR4的蛋白表达显著升高。与模型组比较,逍遥丸组和甘氨酸组TC、TG、ALT含量降低;HDL-C、5-HT、NE、D-木糖排泄率升高;TLR4的蛋白表达降低。结论:非酒精性脂肪肝模型复制成功,符合肝郁脾虚证型,逍遥丸可降低模型大鼠TLR4的表达。

Objective： To observe the effect of xiaoyao pill on the expression of TLR4 in rats with nonalcoholic fatty liver of liver depression and spleen deficiency syndrome. Methods： 32 SD rats of SPF were randomly divided into control group,model group,xiaoyao pill group and glycine group,8 rats in each group. In order to replicate the model,the control group were given basic diet; the other groups were given high fat and sugar diet,eating disorders and chronic restraint stress,a total of 14 weeks. Xiaoyaopill group and glycine group were given respectively xiaoyaopill and glycine gavage on the basis of modeling,1 times / d. After administration for 30 days,the blood was taken from the abdominal aorta. Total serum indexes： cholesterol（ TC）,triglyceride（ TG）,high density lipoprotein（ HDL-C）,transaminase（ ALT）,5-hydroxytryptamine（ 5-HT）,norepinephrine（ NE）,D-xylose excretion rate were detected. The changes in liver morphology was observed with HE staining. The protein expression of TLR4 was detected by Western blotting technique. Results： Compared with the control group,in the model group,TC,TG and ALT levels were significantly increased; HDL-C levels were significantly lower; 5-HT,NE and D-xylose excretion rate were significantly reduced; fat vacuoes were found in the cytoplasm of liver cells; TLR4 protein expression in the liver was significantly higher. Compared with the model group,in the treatment and glycine groups,TC,TG and AL T were decreased; HDL-C,5-HT,NE and D-xylose excretion rate were increased;protein expression of TLR4 were reduced. Conclusion：The animal model of nonalcoholic fatty liver can be replicated successfully,and it is also consistent with liver depression and spleen deficiency type,while xiaoyao pill can reduce the expression of TLR4 in liver of model rats.