DNA扩增过程数据建模方法研究

Study on modeling method of DNA amplification process

在控制过程中,由于在反应室内的环境因素复杂,在循环过程中会随着循环次数的变化引起环温变化,这对最终结果会产生影响.提出了一种因环境变化对控制参数进行调整和修订的数学建模方法,对DNA扩增过程的各个细节利用数学方法进行了分析和研究,构建了数学模型,这种数学模型利用DNA扩增的过程把每次循环的过程控制参数进行了分析和处理,依据DNA本身的特性通过实验方法对控制流程进行了分类和研究.实验结果表明所建立的数学模型在DNA扩增过程中是有效的.

The complexity of the environmental factors in the reaction chamber in the process of the cycle,will affect the final results with the change of the loop temperature.This paper presents a mathematical modeling method for adjusting and revising the control parameters,then the mathematical model is constructed.The process control parameters are analyzed and classified in each cycle according to the characteristics of DNA.The result shows that the mathematical model is effective in the process of DNA amplification.