灰毡毛忍冬种子萌发与幼苗生长影响因子研究

Study on influence factors of seed germination and seeding growth of Lonicera macranthoides

通过研究灰毡毛忍冬种子萌发与幼苗生长影响因子,旨在提高其种子繁殖效率,并为制定种子质量检验方法提供依据。观察灰毡毛忍冬果实和种子形态特征,测定种子吸水量,同时设不同湿沙层积时间、温度和发芽床处理,测定其发芽势、发芽率、发芽指数对种子萌发和幼苗生长的影响。结果表明灰毡毛忍冬种子吸水通透性无障碍,22 h后吸水量接近饱和;4℃湿沙层积处理80 d前,随着层积时间增加种子初始发芽时间缩短,发芽率和发芽势提高,层积100 d,发芽率降低;15℃下,种子发芽和幼苗生长各项指标最佳,其中光照条件下的幼苗子叶宽度显著大于黑暗条件下;种子在纸上、砂上的发芽势、发芽率和发芽指数显著高于其他发芽床,且霉变率低。灰毡毛忍冬的标准发芽试验规程为:种子于4℃湿沙层积80 d,纸上或砂上发芽床,15℃恒温光照培养,首次计数为置床后第4天,末次计数为第23天,统计发芽势的时间为置床后第13天。

In order to improve reproductive efficiency and quality standard,the influence factors of seed germination and seeding growth of Lonicera macranthoides werew studied. The fruit and seed morphological characteristics of L. macranthoides were observed,the seed water absorbing capacity was determined,and different wet sand stratification time,temperature and germination bed treatment were set up. The effects of the parameters on seed germination and seedling growth were analysed. There was no obstacles of water absorption on L. macranthoides seed,quantity for 22 h water absorption was close to saturation. In the first 80 d,with the increase of the stratification time,seed initial germination time was shortened,germination rate and germination potential was improved. Stratification for 100 d,germination rate decreased. At 15 ℃,seed germination and seedling growth indicators were the best. The seedling cotyledon width in light was significantly higher than that in dark. Seeds on the top of paper and top of sand germination rate,germination potential,and germination index was significantly higher than that of other germination bed and mildew rate is low. The optimal conditions of seeds germination test was stratified in 4 ℃ wet sand for 80 d,15 ℃ illuminate culture on the top of paper or top of sand. The first seeding counting time was the 4th day after beginning the test,the final time was the 23 th day. The germination potential statistical time was the 13 th day after beginning the test.