多指标综合评分法优化宣痹安痛方的水提醇沉工艺

Multi-index integrated evaluation method optimizes water-extraction and ethanol precipitation of Xuanbi'antong formula(XBF)

宣痹安痛方为临床治疗冠心病的经验方,该研究确定其最佳水提醇沉工艺参数。采用L9(34)正交设计法对宣痹安痛方进行提取醇沉工艺的研究,以出膏率、丹酚酸B、黄连总生物碱、芍药苷、葛根素、皂苷Rb1、松果菊苷等指标成分含量为考察指标,采用多指标综合加权评分的方法优选出最佳提取醇沉工艺,并对该优选工艺进行验证。结果显示,最佳提取工艺参数为10倍加水量、煎煮时间0.5 h、煎煮3次;最佳醇沉工艺为浓缩液1.12(20℃测),加入乙醇使含醇量达到70%,放置24h。优化后的水提醇沉工艺稳定可靠,可为进一步开发利用该处方提供参考。

To determine the process parameters of optimal water-extraction and ethanol precipitation method for Xuanbi＇ antong（ XBF） extract,which is a clinically experience formula for coronary disease. Orthogonal test L9（ 34） was conducted for the study of XBF water-extraction and ethanol precipitation process. Extractum,salvianolic acid B,rhizoma coptidis alkaloid,paeoniflorin,puerarin,ginsenoside Rb1,ginsenosides and echinacoside were selected as marker components and multi-index comprehensive weighted score was used to select and verify optimal water-extraction and ethanol precipitation process. The optimal extraction process was as follows：XBF was added with 10 times distilled water,decocted for half an hour for 3 times. The best ethanol-precipitation process was established where the ethanol was added up to 70% and precipitated for 24 hours in 1. 12 extract density（ 20 ℃）. The optimized water-extraction and ethanol precipitation method is stable and reliable,and can provide reference for further development and utilization of the formula.