八角莲愈伤组织中黄酮苷类化学成分研究

Flavonoid glycosides from callus cultures of Dysosma versipellis

综合运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、制备薄层色谱和制备型HPLC等多种色谱技术对八角莲愈伤组织的化学成分进行分离纯化,并采用UV,IR,MS和NMR等波谱技术鉴定化合物的结构。从八角莲愈伤组织的95%乙醇提取物中共分离得到7个黄酮苷类化合物,分别鉴定为山柰酚-3-O-[6″-(3'''-甲氧基)-丙二酰基]-β-D-吡喃葡萄糖苷(1),山柰酚-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷(2),山柰素-3-O-β-D-吡喃葡萄糖苷(3),山柰酚-3-O-β-D-吡喃葡萄糖苷(4),异槲皮苷(5),槲皮素-4'-O-β-D-吡喃葡萄糖苷(6)和山柰酚-3-O-(6″-O-丙二酸单酰基)-β-D-吡喃葡萄糖苷(7)。以上化合物均为首次从八角莲愈伤组织中分离得到,其中化合物1,2,3,6和7为首次从八角莲植物材料中分离得到,化合物1是一个新化合物。

Various chromatographic techniques,including silica gel column chromatography,Sephadex LH-20,preparative thinlayer chromatography,and preparative HPLC,were employed to isolate the chemical constituents from callus cultures of Dysosma versipellis. Structures of the compounds were elucidated based on UV,IR,MS and NMR spectroscopic data analysis. Totally,seven flavonoid glycosides were isolated from the 95% ethanol extract of the callus cultures and identified as kaempferol-3-O-[6″-（ 3＇＇＇-methoxy）-malonyl]-β-D-glucopyranoside（ 1）,kaempferol-3-O-（ 6″-O-acetyl）-β-D-glucopyranoside（ 2）,kaempferide-3-O-β-D-glucopyranoside（ 3）,kaempferol-3-O-β-D-glucopyranoside（ 4）,isoquercitrin（ 5）,quercetin-4＇-O-β-D-glucopyranoside（ 6） and kaempferol-3-（ 6″-malonyl）-β-D-glucopyranoside（ 7）,respectively. All these compounds were isolated from callus cultures of D. versipellis for the first time. Compounds 1,2,3,6 and 7 were firstly obtained from plant materials of D. versipellis,and compound 1 was a new compound.