民族药材青阳参的化学成分研究

Chemical constituents of ethnic medicine Cynanchum otophyllum

为提高民族药材青阳参的质量标准,分离纯化质量研究用对照品,采用硅胶柱、ODS开放柱和半制备液相等色谱方法,对民族药材青阳参的乙醇提取物进行分离纯化,通过波谱学方法（MS,NMR）进行结构鉴定。从青阳参的乙醇提取物分离得到12个化合物,包括6个苯乙酮类和6个甾体苷类化合物,分别鉴定为2,4-二羟基-6-甲氧基苯乙酮（1）,4,6-二羟基-2-甲氧基苯乙酮（2）,对羟基苯乙酮（3）,白首乌二苯酮（4）,2,4-二羟基苯乙酮（5）,2,5-二羟基苯乙酮（6）,青阳参苷甲（7）,青阳参苷乙（8）,告达亭-3-O-β-D-加拿大麻吡喃糖-（1→4）-β-D-毛地黄毒吡喃糖苷（9）,告达亭-3-O-β-D-葡萄吡喃糖基-（1→4）-β-D-夹竹桃吡喃糖基-（1→4）-β-D-加拿大麻吡喃糖基-（1→4）毛地黄毒吡喃糖苷（10）,青阳参苷元-3-O-β-D-夹竹桃吡喃糖基-（1→4）-β-D-加拿大麻吡喃糖苷（11）,告达亭-3-O-β-D-夹竹桃吡喃糖基-（1→4）-β-D-加拿大麻吡喃糖基-（1→4）-β-D-加拿大麻吡喃糖苷（12）。化合物1~2为首次从鹅绒藤属植物中分离得到,化合物3~4,9~12为首次从该植物中分离得到的成分。这些化合物是该药材的主要活性成分,为该药材质量标准提高奠定了物质基础。

In order to isolate and purify the reference compounds and improve the quality standard of ethnic medicine of Radix of Cynanchum otophyllum,the ethanol extracts were isolated by column chromatography onsilica gel,C18 reverse-phase silica gel,and semi-preparative HPLC. Twelve compounds were isolated and their structures were elucidated as 2,4-dihydroxy-6-methoxyphenylethanone（ 1）,4,6-dihydroxy-2-methoxyphenylethanone（ 2）, p-hydroxyacetophenone（ 3）, baishouwubenzophenone（ 4）,2,4-dihydroxyacetophenone（ 5）,2,5-dihydroxyacetophenone（ 6）,otophylloside A（ 7）,otophylloside B（ 8）,caudatin-3-O-β-D-cymaropyranosyl-（ 1→4）-β-D-digitoxopyranoside（ 9）,caudatin-3-O-β-D-glucopyranosyl-（ 1 →4）-β-D-oleandropyranosyl-（ 1 →4）-β-D-cymaropyranosyl-（ 1→4）-β-D-digitoxopyranoside（ 10）,qingyangshengenin-3-O-β-D-oleandropyranosyl-（ 1 →4）-β-D-cymaropyranoside（ 11）,caudatin-3-O-β-D-oleandropyranosyl-（ 1→4）-β-D-cymaropyranosyl-（ 1→4）-β-D-cymaropyranoside（ 12） on the basis of spectral analysis.Compounds 1 and 2 were isolated from the genus Cynanchum for the first time,and compounds 3-4,9-12 were obtained from this plant for the first time. These compounds are main active components of Radix of C. otophyllum and can be used as reference substances for the quality control of this ethnic medicine.