结肠慢传输型便秘大鼠肠道菌群的变化及益生菌的干预效果

Changes of intestinal flora and effects of corresponding intervention in colon slow transit constipation rats

目的探讨结肠慢传输型便秘(STC)大鼠肠道菌群变化及益生菌的干预效果。方法采用复方地芬诺酯灌胃的方法建立大鼠STC模型成功后,将32只STC大鼠随机分为STC组(n=16)、STC干预组(n=16),另设正常SD大鼠(n=16)为对照组。利用16S rRNA Real-time PCR技术测定肠道菌群,消化道排空时间采用活性碳灌胃法测定。首先对STC组大鼠粪便肠道菌群进行检测,再根据肠道菌群检测结果对STC大鼠每天灌胃乳酸杆菌及双歧杆菌各0.5 m L,观察干预后实验大鼠粪便主要功能细菌及消化道排空时间变化。结果 STC组和对照组粪便乳酸杆菌、双歧杆菌数分别为4.22±0.07、5.26±0.10和6.51±0.10、7.68±0.17,STC组低于对照组,组间比较差异有统计学意义(P<0.01),粪便总细菌、厚壁菌门、拟杆菌门、梭菌群Ⅳ等主要肠道优势菌群数两组间比较差异无统计学意义(P>0.05)。经乳酸杆菌、双歧杆菌干预后粪便总细菌、厚壁菌门、拟杆菌门、梭菌群Ⅳ3组间比较差异无统计学意义(P>0.05),STC组、STC干预组、对照组乳酸杆菌、双歧杆菌数分别为4.20±0.07、5.21±0.10和6.47±0.09、6.47±0.05和6.50±0.08、7.79±0.06,STC组明显低于STC干预组和对照组,组间比较差异有统计学意义(P<0.01),干预后STC、STC干预组及对照组粪便活性碳排出时间分别为(5.56±0.50)d、(2.55±0.67)d、(0.77±0.01)d,STC组慢于其余两组,组间比较差异有统计学意义(P<0.01)。结论结肠STC大鼠粪便中肠道乳酸杆菌及双歧杆菌数明显减少,主要肠道优势菌群数未发生改变,经乳酸杆菌及双歧杆菌干预后可恢复结肠STC大鼠肠道菌群状态及消化肠道排空时间。

Objective To explore changes of intestinal flora and effects of corresponding intervention in colon slow transit constipation（ STC）. Methods STC model was established by gastric infusion with compound diphenoxylate.Thirty- two STC rats were randomly divided into the STC group（ n = 16） and the intervention group（ n = 16）. Sixteen normal Spragne- Dawley rats were assigned to the control group（ n = 16）. Intestinal flora were detected by 16 SrRNA real-time PCR. Gut emptying time was measured through gastric infusion with activated carbon gastric infusion. Fecal bacteria in the STC group were detected before intervention based on the test results of intestinal flora. Fecal bacteria and gut emptying time were observed after the intervention. Results Fecal lactobacillus,bifidobacterium counts in the STC and control groups were 4. 22 ± 0. 07,5. 26 ± 0. 10 and 6. 51 ± 0. 10,7. 68 ± 0. 17. These bacteria counts were lower in the STC group compared with those in the control group（ P 0. 01）. Counts of total fecal bacteria,firmicutes,bacteroides and clostridium groupⅣ in two groups was not statistically significantly different（ P 0. 05）. After intervention with lactobacillusand bifidobacterium,counts of total fecal bacteria,firmicutes,bacteroides,clostridium group Ⅳ in STC,STC intervention and control group were not statistically significantly different（ P 0. 05）. Lactobacillus and bifidobacterium counts in STC,STC intervention and the control groups were 4. 20 ± 0. 07,5. 21 ± 0. 10 and 6. 47 ± 0. 09,6. 47 ± 0. 05 and 6. 50 ±0. 08,7. 79 ± 0. 06,respectively. Counts in the STC group were significantly lower than in the intervention group and the control group（ P 0. 01）. Gut emptying time in the STC,STC intervention and control groups after intervention were（ 5. 56 ± 0. 50） d,（ 2. 55 ± 0. 67） d,（ 0. 77 ± 0. 01） d. Gut emptying time in STC group was longer than the other two groups（ P 0. 01）. Conclusions Intestinal lactobacillus and bifidobacterium counts in STC rats are reduced. There is no change in main functional bacteria in STC rats. Intervention with lactobacillus and bifidobacterium can restore intestinal microflora and gut emptying times.