血清CA125及HE4水平对卵巢癌组织ERα表达的影响

The effects of serum CA125 and HE4 on the expression of estrogen receptorαon ovarian cancer tissues

目的探讨卵巢癌患者血清CA125及雌激素(HE4)水平对卵巢癌组织雌激素受体α(ERα)表达的影响,为卵巢癌的临床诊断及抗雌激素治疗提供依据。方法收集50例卵巢癌患者手术前血清及手术后组织标本,化学发光法检测卵巢癌患者及正常对照组血清中CA125、HE4含量,免疫组织化学法标示卵巢癌组织中ERα表达状况;并用统计学软件SSPS19.0,比较卵巢癌组织ERα阳性与ERα阴性患者血清中CA125、HE4水平的差异,分析其与ERα表达的关系。结果卵巢癌患者血清CA125及HE4水平均高于正常对照组(P<0.001),且与临床分期有关(P<0.01)。卵巢癌组织ERα阳性与ERα阴性患者血清中CA125水平无明显差异(P=0.360);但HE4水平存在差异(P=0.024)。免疫组化染色显示,卵巢癌组织中ERα阳性率为56%,大多在细胞核表达;其表达水平与血清HE4含量呈负相关(r=0.852,P=0.031)。血清HE4水平相对较低的卵巢癌组织中ERα表达水平较高;而血清HE水平较高的卵巢癌组织中ERα在细胞核表达水平明显降低。结论血清CA125及HE4含量同时增高对卵巢癌诊断具有重要参考价值;血清HE4水平升高可影响ERα表达,其含量高低可为临床以ERα为靶标进行卵巢癌治疗提供依据。

Objective To explore the effects of serum CA125 and Human Epididymis Protein 4（HE4）on the expression of estrogen receptorα（ERα）on ovarian cancer tissues,and provide the basis for diagnosis and treatment of ovarian cancer.Methods The serum levels of CA125 and HE4in 20 cases of health control and 50 cases of patients with ovarian cancer before surgery were measured by chemilumineseent immunoassay（CLIA）.The expression status of ERαon ovarian cancer tissues were detected by immunohistochemical staining.The difference of the serum levels of CA125 and HE4in ERαpositive and negative patients,and their relationship with ERαwere analyzed by SSPS 13.0software.Results Both of the serum levels of CA125 and HE4in ovarian cancer patients were higher than those in health control（P〈0.001）,and related to ovarian cancer clinical stages（P〈0.01）.Although the serum level of CA125 was no difference（P=0.360）,the level of HE4 was difference in ERαpositive and negative patients（P=0.024）.In ovarian cancer tissues,the majority of ERαwas expressed on ovarian epithelial nuclei,its positive rate was 56%.Compared with low level of serum HE4 patients,the nuclear staining for ERαin ovarian cancer tissue from high serum HE4 patients was reduced,and that a negative relationship was showed between the levels of ERαexpression on cancer tissues and serum HE4（r=0.852,P=0.031）.Conclusions The serum levels of CA125 and HE4have an important reference value for diagnosis of ovarian cancer.Especially,due to the increasing of serum HE4 could influence the expression of ERαon ovarian cancer cells,the serum level of HE4 maybe served as an indicator for clinical treatment ovarian cancer using anti-estrogen drug or others targeting the overexpression ERα therapy.