甲状腺癌MHC基因频率分布与可溶性主要组织复合物Ⅰ链相关蛋白A表达的关系

Correlation between MHC gene frequency distribution and s MⅠCA expression in thyroid carcinoma

目的探讨甲状腺癌MHC基因频率分布与可溶性主要组织复合物Ⅰ链相关蛋白A（s MⅠCA）表达的关系。方法采用直接测序法对44例经术后病理证实为甲状腺癌的患者及87例甲状腺良性肿瘤患者、133例健康体检者全血进行MHC（HLA、MⅠCA、MⅠCB）高分辨分型,采用Pypop Win32进行MHC系统频率分析,SPSS 20.0统计软件分析甲状腺癌与MⅠCA/B及HLA等位基因频率的相关性,运用ELISA对上述对象血清进行s MⅠCA半定量检测。结果 （1）MⅠCB＊004：01为甲状腺癌的保护等位基因[P=0.014,RR（95%CI）=0.122（0.016~0.91）];（2）DRB1＊15：01和DRB1＊04：03为甲状腺良性肿瘤的保护等位基因（P〈0.05,RR〈1）;（3）甲状腺癌患者与甲状腺良性肿瘤及健康体检者比较,血清s MⅠCA水平差异无统计学意义,其中甲状腺癌患者血清s MⅠCA水平为（0.233 1±0.244 5）ng/m L。结论 MHC系统基因与甲状腺癌存在关联,MⅠCB＊004：01与患甲状腺癌风险相关。

Objective To investigate the MHC gene frequency distribution and sMⅠCA expression levels in thyroid carcinoma,as well as their correlation. Methods Forty- four patients with thyroid carcinoma,87 with benign thyroid nodules and 133 healthy individuals were enrolled and whole blood sample was collected for each subject. The MHC（ HLA,MⅠCA and MⅠCB） alleles were identified by SBT. Gene frequency was analyzed by Py Pop Win32 software.SPSS20. 0 was used to analyze the correlation of cancer with MⅠCA / B and HLA allelic frequency. ELISA was applied to semi- quantitatively measure the serum concentration of soluble MⅠCA（ sMⅠCA）. Results（ 1） M Ⅰ CB ＊ 004： 01 was a protective factor from thyroid carcinoma [P = 0. 014,RR（ 95% CI） = 0. 122（ 0. 016 ~ 0. 91） ];（ 2） DRB1＊ 15： 01 and DRB1＊ 04： 03 were protective alleles of benign thyroid nodules（ P 0. 05,RR 1）;（ 3） There was no significant difference among patients with thyroid carcinoma,benign thyroid nodules and healthy individuals in the level of s MⅠCA.The mean s MⅠCA level of thyroid carcinoma patients is（ 0. 233 1 ± 0. 244 5） ng/mL. Conclusion MHC system is associated with thyroid carcinoma,of which MⅠCB＊ 004： 01 is correlated with risks of thyroid carcinoma.