电针治疗对兔膝骨关节炎软骨的影响

Effects of electroacupuncture on cartilage of rabbits with knee osteoarthritis

目的初步探讨电针对兔膝骨关节炎(osteoarthritis,OA)模型软骨细胞修复的影响及作用机制。方法健康新西兰大白兔(雄性)60只,随机分为正常对照组,模型组和电针治疗组,每组20只,模型组和电针治疗组均采用伸直位管型石膏固定左膝关节,正常对照组和模型组于喂养6周后取内侧髁软骨观察造模后相关指标变化,治疗组在进行电针治疗15 d后以同样方法观察治疗后相关指标改变情况:软骨细胞形态、蛋白多糖和胶原等基质含量变化。结果正常对照组左膝关节无明显关节积液;模型组左膝关节大部分出现关节积液;电针治疗组大部分动物出现关节积液及滑膜增生。正常对照组软骨表面平整;模型组软骨表层出现溃烂缺失,甲苯胺蓝染色明显减退;电针治疗组软骨细胞数明显增多,甲苯胺蓝染色轻度减退。模型组软骨结构,软骨细胞和潮线完整性评分与电针治疗组比较,差异有统计学意义(P<0.05)。正常对照组Mankin's甲苯胺蓝评分与模型组比较,差异有统计学意义(P<0.05),模型组与电针治疗组比较,差异有统计学意义(P<0.05)。结论采用伸直位管型石膏固定法制作兔膝OA模型能较好地模拟OA的发病过程。电针能有效治疗膝OA,有利于软骨细胞的修复。

Objectives To observe the effects of electroacupuncture（ EA） on chondrocyte repairing of rabbits with knee osteoarthritis（ OA）,and to explore its action mechanism. Methods Sixty male New Zealand white rabbits were randomly divided into control group,model group and EA treatment group,with 20 rabbits in each group. The left knees of rabbits in model group and EA treatment group were immobilized in full extention with plaster casts for 6 weeks to establish OA models. After modeling and treatment for 15 days in EA treatment group, the related indexes including chondrocyte morphology,the content of proteoglycan and collagen matrix,the expression levels of IL-1β in femoral medial condyle cartilage were observed and detected. Results There was no obvious joint effusion in left knees of rabbits in control group,however,which appeared in most rabbits of model group and EA treatment group,with synovial hyperplasia. Cartilage surfaces in control group were smooth,but fester and defection were observed in cartilage surfaces of rabbits of model group,furthermore,toluidine blue staining was obviously destained. The chondrocyte numbers in EA treatment group were obviously increased and toluidine blue staining was slightly decreased. There were significant differences in cartilaginous structure, integrality scores of chondrocyte and tidal line between control group and EA treatment group（ P〈0. 05）. There were significant differences in Mankin＇s toluidine blue scores between control group and EA treatment group as well as between model group and EA treatment group（ P〈0. 05）. Conclusion The OA models established by immobilizing knee in full extention with plaster casts can well simulate the pathogenesis of OA. Electroacupuncture can promote the repairing of cartilage cells,thus,which can effectively treat knee osteoarthritis.