摘要
目的:探讨上皮性卵巢癌(epithelial ovarian cancer,EOC)中聚腺苷二磷酸核糖聚合酶-1[poly(ADP-ribose)polymerase-1,PARP-1]的表达及其与上皮-间质转化(epithelial-mesenchymal transition,EMT)的关系。方法:免疫组化、实时荧光定量PCR法检测EOC和良性卵巢肿瘤组织中PARP-1、E-钙黏蛋白(E-cadherin)、波形蛋白(vimentin)和转录调控因子Snail的表达;Western blotting法检测高效PARP-1抑制剂PJ34处理SKOV3细胞后PARP-1、E-cadherin、vimentin和Snail蛋白的表达。结果:PARP-1、vimentin和Snail在EOC中阳性表达率高于良性卵巢肿瘤组织,而E-cadherin则相反,差异均有统计学显著性(P<0.05)。PARP-1、E-cadherin、vimentin和Snail与EOC的病理分级、临床分期和有无淋巴结转移有关(P<0.05),与年龄和病理类型无关。E-cadherin表达与PARP-1表达呈负相关(P<0.05),vimentin、Snail表达与PARP-1表达呈正相关(P<0.05)。EOC中PARP-1、vimentin和Snail mRNA的相对表达量高于良性卵巢肿瘤组织,E-cadherin mRNA的相对表达量低于良性卵巢肿瘤组织,差异均具有统计学显著性(P<0.05)。PJ34处理SKOV3细胞后,PARP-1、vimentin和Snail的蛋白水平明显下降,E-cadherin的蛋白水平显著提高,差异有统计学显著性(P<0.05)。结论:PARP-1通过调控E-cadherin、vimentin和Snail的表达促进EOC上皮间质转化。PARP-1及其参与的上皮-间质转化在EOC进展中发挥重要作用。
AIM:To investigate the expression of poly( ADP-ribose) polymerase-1( PARP-1) in the epithelial ovarian cancer( EOC) and its relationship with epithelial-mesenchymal transition( EMT).METHODS:The expression of PARP-1,E-cadherin,vimentin and Snail was detected in the EOC and benign ovarian tumor tissues by immunohistochemical method and real-time PCR.The expression of PARP-1,E-cadherin,vimentin and Snail proteins in the SKOV3 cells treated with efficient PARP-1 inhibitor PJ34 was determined by Western blotting.RESULTS:The positive expression rates of PARP-1,vimentin and Snail were significantly higher in the EOC than that in the benign ovarian tumor tissues,whereas the positive expression rate of E-cadherin was the opposite( P〈0.05).The expression of PARP-1,E-cadherin,vimentin and Snail in the EOC was associated with the histological grade,clinical stage and lymphatic metastasis( P〈0.05),but no relationship with age and pathological types was observed.The expression of E-cadherin in the EOC was negatively corelated to that of PARP-1.In contrast,the expression of vimentin and Snail in the EOC was positively co-related to that of PARP-1.The relative mRNA expression of PARP-1,vimentin and Snail in the EOC was significantly higher than that in the benign ovarian tumor tissues( P〈0.05),while the mRNA expression of E-cadherin in the EOC was remarkably lower than that in the benign ovarian tumor tissues( P〈0.05).The protein expression of PARP-1,vimentin and Snail in the SKOV3 cells was significantly decreased( P〈0.05),while E-cadherin protein was increased after treated with PJ34( P〈0.05).CONCLUSION:PARP-1 may contribute to the onset of EMT in the EOC by regulating the expression of E-cadherin,vimentin and Snail.The role of PARP-1,which is relevant to EMT,might be important in the development of ovarian cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第3期425-431,共7页
Chinese Journal of Pathophysiology
基金
国家自然科学基金应急管理项目(No.81441075)
山东省自然科学基金资助项目(No.ZR2014HM108)
山东省自然科学基金青年项目(No.ZR2013HQ030)