重组大肠杆菌表达天蓝色链霉菌M145 narG2H2J2I2基因还原硝酸盐能力研究

Study on the Ability of Nitrate Reduction in Recombinant Escherichia coli Strain with Streptomyces coelicolor M145 narG2H2J2I2 Gene

将天蓝色链霉菌M145 narG2 H2J2I2基因转入大肠杆菌中表达,检测重组大肠杆菌在有氧和微氧环境下生长和消耗硝酸盐的能力.结果表明,在有氧和微氧环境下,大肠杆菌NE01生长最慢、DH10B生长最快,而消耗硝酸盐速度方面却为NE01最快,NE02最慢;在微氧环境下DH10B、NE02、NE01消耗硝酸盐速度都较其有氧环境下快,且于10h内NE01将硝酸盐消耗完毕.因此,有氧和微氧环境下,重组大肠杆菌克隆表达天蓝色链霉菌M145 narG2 H2J2I2基因能促进硝酸盐消耗,且在微氧环境下消耗得更快.本研究结果为继续改造重组菌用于硝酸盐污染的环境修复奠定了基础.

To study the expression of the Streptomyces coelicolor M145 narG2 H 2 J 2 I2 gene in Escherichia coli and the influence in nitrate metabolism, narG2H2J212 gene was transformed to E. coli to detect the ability of recombinant E. coli growth and consumption of nitrate under aerobic and micro-oxygen condition. The growth curve indicated that NE01 grows much slower than NE02 and the growth of DH10B under aerobic and microaerophilic conditions. However, for the ability of reduction of nitrate, the order is NE01, DH10B and NE02. Specifically, the ability of the strains DH10B, NE02, NE01 to deoxidize the nitrate is more efficient at microaerophilie condition compared to the strains at aerobic condition. Furthermore, the ability of recombinant strain NE01 to deoxidize nitrate seems more faster than DH10B and NE02. In conclusion, the recombinant E. coli with inducible S. coelicolor M145 narG2H2J212 gene can promote the ability to deoxidize nitrate under aerobic and microaerophilic conditions. This study may contribute to the future treatment of nitrate pollution using the recombinant E. coli.