注意缺陷多动障碍大鼠模型的建造

Construction of rat models of attention deficit hyperactivity disorder

目的:探讨注意缺陷多动障碍大鼠模型的建造技术。方法:将20只4周龄SD大鼠随机分成模型组和对照组,模型组自由饮用0.2 mg/m L醋酸铅溶液。28 d后运用开场实验、Morris水迷宫、Lat迷宫观察两组大鼠行为学表现;采用ICP-MS检测大鼠血铅浓度、酶联免疫吸附测定法法(ELISA)测定大鼠血清中多巴胺和去甲肾上腺素含量。结果:模型组在开场实验中大鼠穿越格子数、直立次数增多(P<0.05),Lat迷宫中直立次数及穿越角落的时间比例增加(P<0.05),Morris水迷宫目标象限停留时间缩短(P<0.05);模型组大鼠血铅浓度高于对照组(P<0.05),血清多巴胺和去甲肾上腺素含量低于对照组,差异有统计学意义(P<0.05)。结论:采用自由饮用0.2 mg/m L醋酸铅溶液法成功建造了ADHD大鼠模型,具有ADHD基本临床特征,可做为ADHD的动物模型进行实验研究。

Objective： To explore the method of constructing rat models of attention deficit hyperactivity disorder（ ADHD）. Methods： 20 SD rats were randomly divided into the model group and the control group with the model group drinking 0. 2 mg / m L solution of lead acetate. 28 days later,the rats＇ behavioral manifestations were observed by using the open field,the Morris water maze and the Lat maze; the rats＇ lead concentration in the blood was determined by using ICP- MS; the contents of dopamine and norepinephrine in the rats＇ serum were determined by using enzyme linked immune sorbent assay（ ELISA）. Results： The occasions of the rats＇ in the model group going through the lattice standing up were more and more frequent in the open field（ P〈0. 05）; the occasions of the rats＇ standing upright were more frequent and the time for the rats to go across the corner was lengthened in the Lat maze（ P〈0. 05）; the target quadrant time in the Morris water maze was shortened（ P〈0. 05）; the lead concentration in blood of the model group was significantly higher than that of the control group（ P〈0. 05）; the contents of dopamine and norepinephrine in the serum of the model group were lower than those of the control group,the difference being significant（ P〈0. 05）. Conclusion：Rat models of ADHD can be constructed successful by freely drinking 0. 2 mg / m L solution of lead acetate,which have the basic clinical characteristics of ADHD and can be studied as animal models of ADHD.