利用RNA-Sequencing鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因

Identification of differentially expressed genes in rat model of cerebral ischemia-reperfusion injury by RNA-Sequencing

目的利用RNA-Sequencing方法,试图鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因,为探讨脑缺血-再灌注损伤的分子机制提供实验依据。方法首先利用RNA-Sequencing方法初步鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因,然后利用生物信息学方法对差异表达基因的功能进行预测分析;再利用RT-PCR和qRT-PCR方法进一步检测差异基因的表达变化。结果与对照组相比,脑缺血-再灌注损伤实验组共鉴定出182个基因差异表达,其中156个基因表达上调,26个基因表达下调;RT-PCR和qRT-PCR方法进一步验证其中3个基因表达上调,2个基因表达下调。结论利用RNA-Sequencing方法可以鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因,表明这些基因可能参与了脑缺血-再灌注损伤,为进一步揭示脑缺血-再灌注损伤机制奠定了实验基础。

Objective To identify differentially expressed genes in rat model of cerebral ischemia reperfusion injury using RNA-Sequencing and elaborate the molecular mechanism of cerebral ischemia reperfusion injury. Methods RNA-Sequencing was used to identify differentially expressed genes. Bioinformatics methods were used to predict the function of differentially expressed genes. RT-PCR and qRT-PCR were used to further detect the expression of different genes. Results Compared to control,182 genes were identified differentially expressed in model of ischemia-reperfusion injury. 156 genes were up-regulated and 26 genes were down-regulated. The expression of three genes were further proved to increase and two genes decrease using RT-PCR and qRT-PCR. Conclusion RNA-Sequencing could identify differentially expressed genes in rat model of cerebral ischemia-reperfusion injury suggesting that these genes may participate in cerebral ischemiareperfusion injury. This study provides a basis to further reveal the mechanism of cerebral ischemiareperfusion injury.