农杆菌遗传转化西瓜的影响因素及应用研究进展

Influencing factors and research progress on transformation of watermelon by Agrobacterium

农杆菌介导的遗传转化是西瓜基因工程的常用方法。在介绍农杆菌介导西瓜遗传转化影响因素和西瓜转基因应用的基础上,主要分析了影响农杆菌介导西瓜转化效率的重要因素,包括农杆菌菌株和载体类型、无菌苗苗龄、外植体类型、预培养时间、共培养时间、农杆菌侵染浓度和时间、抗生素的种类和浓度及乙酰丁香酮等,同时总结了西瓜转基因研究的多种应用价值及研究成果。

Agrobacterium-mediated genetic transformation was the commonly used method in the watermelon genetic engineering. On the basis of an introduction about the influencing factors of Agrobacterium-mediated transformation of watermelon and the application of transgenic watermelon,this study summarized the major influencing factors in genetic transformation process of watermelon,such as the strain of Agrobacterium,the type of vector,the age of seedling,the type of explants,pre-culture time,co-culture time,the concentration and time of infection,the type and concentration of antibiotics and the concentration of acetosyringone. The application value and achievement of transgenic watermelon were also reviewed.