一株冬虫夏草相关菌株的生物学特性

Biological characteristics of a strain of Ophicordyceps sinensis related fungi

从冬虫夏草虫体上分离得到一株真菌,对其生物学特性和侵染力,及其与冬虫夏草菌的相互关系进行了初步研究,并对其进行了分子鉴定。结果表明,该菌丝生长的最适温度为20-23℃,最适p H为7.50,最适碳氮源分别是蔗糖和麸皮,对蝙蝠蛾幼虫的侵染力为100%,并在与冬虫夏草菌平板对峙生长中发现其对冬虫夏草菌的生长抑制率高达43.37%。PCR结果显示,从菌丝体中扩增获得了560 bp左右的一段ITS序列,系统发育分析表明,该菌是白僵菌,Gen Bank登录号为KP284435。

A preliminary study was carried out to investigate the biological characteristics,infection rate of a fungus isolated from Ophicordyceps sinensis,as well as its relationship with Ophicordyceps sinensis,and the molecular identification was analyzed. The results showed that the optimum temperature and p H for culture was 20 to 23 ℃ and 7. 50 respectively. The optimum carbon sources and nitrogen sources was sucrose and bran. The infection rate on Hepialidae larva was 100%. The inhibition rate on Ophicordyceps sinensis was 43. 37% in flat confrontation test. PCR results showed that about 560 bp ITS fragments were amplified from the mycelium. Phylogenetic analysis indicated that the fungi belonged to Beauveria,and Gen Bank accession number is KP284435.