重金属Cr^(6+)耐受细菌的筛选研究

Screening of heavy metal Cr^(6+) resistant strains

土壤中重金属Cr6+污染日益严重,其对植物的影响也越来越大,并可通过食物链富集进入人和动物体内,从而危害人畜健康。试验在分别添加0.3和0.5 g·kg-1Cr6+的土样中进行耐性菌株的筛选,以期为微生物修复重金属污染提供技术支持。结果表明,该研究共获得了9株具有较好Cr6+耐性的菌株。它们均可在含0.5,1.0 mmol·L-1Cr6+的LB培养基中生长。其中,编号为J3,J5,J8的菌种耐性达到2 mmol·L-1,在含2 mmol·L-1Cr6+的液体培养基中培养48 h后,培养基中的Cr6+含量分别降低23%,36%,7%。经测定,上述3种菌分别为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、地衣芽孢杆菌(Bacillus licheniformis)、沙福芽孢杆菌(Bacillus sonorensis)。

Cr^6＋is a hazardous heavy metal element to plant growth,and can endanger human and animal health through food chain enrichment. In the present study,Cr^6＋resistant strains were screened from soils added with 0. 3,0. 5 g·kg^- 1Cr^6＋,in order to provide technical support for microbial remediation of heavy metal pollution. It was shown that 9 Cr^6＋resistant strains were screened,all of which could grow normally in media with 0. 5,1. 0 mmol·L^- 1Cr^6＋. Among them,strains named as J3,J5 and J8 were able to grow in media with 2 mmol·L^- 1Cr^6＋. Besides,after 48 h cultivation in media with 2 mmol·L^- 1Cr^6＋,Cr^6＋content in media with J3,J5,J8 was reduced by23%,36%,7%,respectively. Sequencing analysis showed that J3,J5,J8 were Bacillus amyloliquefaciens,Bacillus licheniformis,Bacillus sonorensis,respectively.