一种牵张成骨大鼠实验模型的建立

Establishment of a novel rat model of distraction osteogenesis

目的建立一种新型SD大鼠胫骨牵张成骨模型的方法。方法 40只雄性SD大鼠右侧胫骨截骨并短缩4 mm,以自行研究设计的环状牵张外固定器固定,于骨折修复期予以牵张外固定器恢复胫骨高度,术后通过大体观察、影像学观察以及组织形态学方法观察截骨部位的愈合与恢复情况。结果术后外固定器稳定有效,对大鼠功能活动无明显影响。胫骨高度恢复,骨性愈合。结论实验所建立的大鼠胫骨牵张成骨模型可靠实用,具有可重复性。

Objective To establish a new model of distraction osteogenesis in rat tibia. Methods A customized external fixation device was attached to the right tibia of 40 male Sprague-Dawley rats. A 4-mm bone block was resected and the tibia was shortened. To investigate the bone healing during the distraction osteogenesis period and reconstruction period,and general observation,x-ray analysis,and histological examination were performed. Results The device was stable and effective without hindrance. The tibia length was recovered and bone union was achieved. Conclusions A novel Sprague-Dawley rat model of distraction osteogenesis in tibia is successfully established,and this is a reliable,practical and reproducible model.