益气补肾方药对顺铂致大鼠肾毒性及顺铂抗肿瘤活性的影响

Effects of herbs on cisplatin-induced nephrotoxicity and on cisplatin anticancer activity in rats

目的:探讨益气补肾方药对顺铂(DDP)致大鼠肾损伤及DDP抗肿瘤活性的影响。方法:实验大鼠一次性尾静脉注射DDP(8 mg/kg)建立DDP损伤模型,分别用低剂量(10.8 g/kg)、中剂量(21.6 g/kg)和高剂量(32.4 g/kg)中药进行保护,通过检测大鼠肾脏系数、肾组织Pt含量、血清尿素氮(BUN)和肌酐(Cr)含量探讨不同浓度益气补肾方药对DDP致大鼠肾损伤的保护作用。昆明小鼠接种S180瘤株建立小鼠肿瘤模型,一次性尾静脉注射DDP(8 mg/kg),通过测定肿瘤抑制率探讨不同浓度益气补肾方药对DDP抗肿瘤活性的影响。结果:益气补肾方药可以有效降低DDP引起的大鼠肾脏系数、肾组织Pt含量、血清BUN和Cr含量的增高。DDP可显著抑制荷瘤S180小鼠肿瘤的生长,益气补肾方药对DDP的肿瘤抑制率没有显著性的影响。结论:适当剂量的益气补肾方药可以有效保护DDP引起的肾损伤,且不影响DDP的抗肿瘤活性。

AIM： To observe the effects of different doses of herbs on cisplatin（ DDP）-induced nephrotoxicity and on DDP anticancer activity in rats.METHODS： DDP nephrotoxicity model was caused by a single dose of DDP（8mg/kg）,and then rats in herbs groups were treated with low dose herbs（10.8 g/kg）, medium dose herbs（21.6 g/kg） and high dose herbs（32.4 g/kg） respectively.The protective effect of different dose herbs against DDP-induced nephrotoxicity in rats were measured by kidney coefficient,platinum accumulation,serum urea nitrogen（ BUN） and creatinine（ Cr） level.Tumor bearing mouse model was established by S180 cells rising in Kunming mouse.After a single dose of DDP（8mg/kg） injection,effects of different doses of herbs on DDP anticancer activity were measured by tumor inhibition rates.RESULTS： Herbs could obviously reduce kidney coefficient, platinum accumulation, BUN and Cr level,which were enhanced by DDP.DDP could significantly inhibit tumor growth in mice,and herbs had no significant effect on the tumor inhibition rates of DDP.CONCLUSION： Proper dose of the herbs can effectively antagonize nephrotoxicity induced by DDP and have no disturb on DDP anticancer activity.