一株DBP高效降解菌的分离、鉴定与降解性能

Isolation and identification of highly efficient DBP-degrading strain and its degradation study

从镇江某垃圾站污染土壤中分离出1株能够以邻苯二甲酸二丁酯为唯一碳源和能源生长的细菌高效降解菌TM。经形态观察、生化鉴定、16S rRNA序列及系统发育分析,鉴定该菌株为变形假单胞菌(Pseudomonas plecoglossicida)。采用正交实验和单因素对照实验对这株菌株的降解条件进行优化,确定其最适生长条件为:温度30℃,p H=7.0。在最适降解条件下,其在72 h内对400 mg/L DBP降解率达到88.56%,为邻苯二甲酸二丁酯的高效降解菌。底物广谱性实验表明,该菌株邻苯二甲酸二辛脂(DOP)、邻苯二甲酸(2-乙基已基)酯(DEHP)都具有良好的降解能力,表明其具备良好的底物广谱性,说明该菌株在处理邻苯二甲酸酯类化合物的污染治理中有独特的应用潜力。

A bacterial strain TM that used dibutyl phthalate as the sole carbon and energy source was isolated from contaminated soil of a garbage station in Zhenjiang,China. Based on its morphology,physiological characteristics,and 16 S r DNA sequence analysis,this strain was identified as Pseudomonas plecoglossicida. Single-factor and orthogonal tests showed that optimum temperature and pH for the growth of and styrene degradation by this strain were 30 ℃ and 7. 0,respectively. Under optimal conditions,the strain degraded 〉88. 56% of400 mg / L DBP within 72 h. Use of diverse degradable substrates showed that the strain could efficiently use other phthalate esters such as di-n-octyl phthalate and bis（ 2-ethylhexyl） phthalate. Thus,our results indicated that the TM strain could be potentially used for eliminating pollution caused by phthalate esters.