儿童支气管哮喘与肺炎支原体感染的相关性研究

Research of the Correlation of Bronchial Asthma in Children with Mycoplasma Pneumoniae Infection

目的：探究儿童支气管哮喘与肺炎支原体感染的相关性。方法：选取2012年5月-2015年5月在本院进行支气管哮喘疾病治疗的80例患儿作为观察组,另外选取同期的80例上呼吸道感染患儿作为对照组。采用酶联免疫吸附试验法测定MP-Ig M,以MP-Ig M滴度大于1∶40为阳性,表示近期有肺炎支原体感染,在Bio-Rad450型酶标仪上运用ELISA法测定血清中的s IL-2R和IL-4的水平,对两组患儿的检测结果进行比较。结果：观察组的肺炎支原体感染的阳性率为42.50%（34/80）,明显高于对照组的16.25%（13/80）,差异有统计学意义（P〈0.05）。观察组血清总Ig E和外周血EOS绝对值分别为（389.42±210.22）U/m L和（519.35±45.70）×10^6/L,均显著高于对照组的（140.12±58.30）U/m L和（350.46±35.47）×10^6/L,差异均有统计学意义（P〈0.05）。观察组的s IL-2R及IL-4分别为（483.5±43.8）ng/L和（33.6±3.1）ng/L,均明显高于对照组的（206.8±18.9）ng/L和（8.9±0.5）ng/L,差异均有统计学意义（P〈0.05）。结论：儿童支气管哮喘与肺炎支原体感染之间一定存在十分紧密的关系,所以在治疗支气管哮喘的患儿时,医生应该要考虑到其肺炎支原体感染的可能性,以便尽快找到诊断依据,同时应该尽早尽快地诊断并治疗支气管哮喘患儿。

Objective：To explore the correlation of bronchial asthma in children with mycoplasma pneumoniae infection.Method：Eighty children with bronchial asthma disease admitted to our hospital from May 2012 to May 2015 were selected as the observation group,while the other 80 children with respiratory infections in the same period in our hospital were chosen as the control group.Enzyme-linked immunosorbent assay（ELISA） was used to determine the MP-Ig M,which was considered positive for the mycoplasma pneumoniae infection when over the titers of 1∶40.ELISA method was used to determine serum s IL-2R and IL-4 levels in the Bio-Rad450 microplate reader.The results of the two groups were compared after the detection.Result：The positive rate of MP-Ig M in the observation group was 42.50%（34/80）,which was significantly higher than 16.25%（13/80） in the control group,the difference was statistically significant（P〈0.05）.The total serum Ig E and peripheral blood EOS absolute values in the observation group were（389.42±210.22）U/m L and（519.35±45.70）×10^6/L,which were significantly higher than（140.12±58.30）U/m L and（350.46±35.47）×10^6/L in the control group,the differences were statistically significant（P〈0.05）.The s IL-2R and IL-4 of children in the observation group were（483.5±43.8）ng/L and（33.6±3.1）ng/L,which were significantly higher than（206.8±18.9）ng/L and（8.9±0.5）ng/L in the control group,the differences were statistically significant（P〈0.05）.Conclusion：Mycoplasma pneumoniae infection has a certain close relationship with children＇s bronchial asthma,so the possibility of mycoplasma pneumoniae infection should be considered in the treatment of children with bronchial asthma for diagnostic evidence,and the diagnosis and treatment for children with asthma should be as early as possible.