海螵蛸/外消旋聚乳酸复合人工骨材料的制备及表征

Preparation and characterization of cuttlebone/racemic polylactic acid composite artificial bone materials

背景:海螵蛸富含钙元素,与自然骨组织有天然的亲和性,但其单独作为支撑材料应用时脆性较大、强度较低。目的:自主研发海螵蛸/外消旋聚乳酸复合人工骨组织支架材料,并检测其各项性能。方法:采用盐析法将海螵蛸和外消旋聚乳酸分别以1∶2、2∶1、1∶1的质量比组合,制备成复合人工骨,以单纯海螵蛸为对照,检测4种材料的微观结构、孔隙率、水结合力、生物力学及体外降解性能。结果与结论:海螵蛸表面棘状起,纹理清晰,多孔状;复合人工骨类似松质骨,大孔孔径500-1 500μm,小孔孔径150-300μm,微孔较少,孔间连通性好。3种复合人工骨的抗弯强度、弹性模量、体外降解性能均优于海螵蛸(P<0.05),孔隙率低于海螵蛸(P<0.05),复合人工骨组间上述指标比较差异均无显著性意义;4组间水结合力比较差异无显著性意义。表明海螵蛸/外消旋聚乳酸复合人工骨具有良好的微观结构、力学性能与降解性能。

BACKGROUND： Cuttlebone which is rich in calcium has a natural affinity with the nature bone tissue. However, when the cuttlebone is applied as supporting material independently, its brittleness is larger and the intensity is lower.OBJECTIVE： To independently research and develop cuttlebone/racemic polylactic acid composite artificial bone tissue scaffolds, and detect their various performance. METHODS： The composite artificial bone was prepared by combining the cuttlebone and racemic polylactic acid at the weight ratio of 1：2, 2：1 and 1：1 using salting out method. The pure cuttlebone was taken as control. The microstructure, porosity, water binding force, biological mechanics and in vitro degradation performance were determined. RESULTS AND CONCLUSION： The surface of cuttlebone had spine shape, clear texture and cellular distribution. Composite artificial bone was similar to the cancellous bone, whose big pore diameter was 500-1 500 μm and small pore diameter was 150-300 μm. Composite artificial bone had less micropores, and the connectivity between the holes is good. The bending strength, the elastic modulus, in vitro degradation properties of these three kinds of composite artificial bone were better compared with cuttlebone（P 〈0.05）, porosity was less than that of cuttlebone（P 〈0.05）, and there were no significant differences between these composite artificial bone groups. There was no significant difference in the water binding force between these four groups. These results demonstrate that cuttlebone/racemic polylactic acid composite artificial bone has good microstructure, mechanical properties and degradation performance.