体积分数95%乙醇建立薄型子宫模型大鼠子宫内膜中波蛋白和血管内皮生长因子的变化

Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol

背景:乙醇化学损伤法能够有效对大鼠子宫进行薄型子宫内膜建模,薄型子宫内膜增殖相关蛋白表达减少,有助于研究薄型子宫内膜相关发生机制,为临床治疗提供指导。目的:探索高浓度乙醇致薄型子宫后子宫内膜中波蛋白和血管内皮生长因子的表达变化。方法:SD雌鼠右侧子宫缓慢注入0.3 mL体积分数95%乙醇建立薄型子宫模型,左侧子宫注入等量生理盐水作为对照侧。结果与结论:与对照侧相比,大鼠损伤侧子宫内膜明显变薄,腺体数量减少,结构紊乱,波蛋白和血管内皮生长因子表达明显降低。说明体积分数95%乙醇损伤可有效建立大鼠薄型子宫内膜动物模型,且与增殖相关的蛋白在薄型子宫内膜中表达出现变化。

BACKGROUND： Ethanol chemical damage method can be used to establish thin endometrium rat model. Expression of proliferation-related proteins is decreased in thin endometrium animal model, which contributes to the study of thin endometrium-related mechanism and provides guidance for clinical treatment. OBJECTIVE： To explore the changes in vimentin and vascular endothelial growth factor expression in rat modelsof high concentrations of ethanol-induced thin endometrium. METHODS： 0.3 mL 95% ethanol was slowly injected in the right uterus of female Sprague-Dawley rats to establish rat models of thin endometrium. An equal volume of physiological saline was injected into the left uterus as control sides. RESULTS AND CONCLUSION： The thickness of endometrium in the injured uterus was significantly thinner than the control ones. Number of glands reduced, structure became disorder, and vimentin and vascular endothelial growth factor expression markedly diminished. These results suggest that 95% ethanol injury can effectively establish rat models of thin endometrium. Proliferation-related protein expression altered in the thin endometrium.