生物样本库质量控制体系在冠心病中的初步应用

Discussion of Biosample quality management in CAD Biobank

目的探讨老年病生物样本库建立质量控制体系在老年冠心病中的应用研究。方法制定老年病样本库操作流程及质量控制体系,规范化收集、处理及保存诊断为老年冠心病的血液标本380例,进行生化指标测定、血浆及全血基因组DNA提取,并检测DNA的浓度和纯度,同时建立一套信息管理系统用于老年冠心病生物样本库的管理。结果 380例有完整临床资料的老年冠心病患者的全血标本、血浆及DNA的提取成功率均为100%,并随机抽取20份DNA进行质量检测,结果表明所收集标本质量较好。初步建立了规范化的样本信息资料软件管理系统。结论该研究有效的在老年冠心病中建立了标准化的质量控制系统并加以应用研究,为冠心病研究提供规范化的临床样本资源平台。

Objective To discuss the establishment of standardized operation procedure and quality control system of blood specimens from patients with CAD in Biobank. Methods An information management system and the operational processes system in CAD Biobank for blood sample collection, quality control system were developed. Whole blood samples were collected from 380 patients whom were all diagnosed with CAD. Plasma and whole blood genomic DNA was extracted from the specimens, and the purity and concentration of DNA were determined. Results Blood samples of 380 CAD patients were collected. 20 were randomly selected for DNA and RNA quality control testing. The success rate was 100% for extraction of plasma and whole -blood genomic DNA. The results showed these collected specimens had high quality. Conclusion The quality control system of CAD Biobank has been successfully established in this study. Development of standardized information management and quality control of specimens is helpful to improve the efficiency of CAD sample quality