摘要
为了检测苦苣苔科植物的基因组大小多样性并进一步补充该科植物的DNA C值数据库,首次采用流式细胞术对中国苦苣苔科13属34种5变种的基因组大小进行了测定和分析。结果表明:所测物种中白花蛛毛苣苔的基因组最小,1C值平均为0.62±0.03pg,小石蝴蝶的基因组最大,1C值平均为2.54±0.04pg,其他物种的1C值均介于0.62-2.54pg之间。新近修订的石山苣苔属、报春苣苔属和半蒴苣苔属中分别至少有5个物种的1C值被测定,其中石山苣苔属的基因组大小多样性最高,与该属具有较高的形态多样性相一致。截至目前,已测定和报道DNA C值的苦苣苔科植物共计18属151种5变种,1C值在科水平上变化了近4.1倍。根据Soltis对基因组大小的分类方法,苦苣苔科植物的基因组属于小或极小基因组。
In order to detect genome size diversity in Gesneriaceae and further complement plant DNA C-values database,DNA C- values were determined for 34 species and 5 subspecies of 13 genera in Chinese Gesneriaceae for the first time using flow cytometry. The results showed that haploid DNA content( 1C-value) of species included in this study varied from 0. 62 pg in Paraboea martini to 2. 54 pg in Petrocosmea minor,with all other species falling in this range. For recently redefined genus of Petrocodon,Primulina and Hemiboea,genome size of at least five species were estimated; however,1C- values indicated that Petrocodon has the highest genome size diversity,which is consistent with the high morphological diversity in this genus. Until now,DNA C- values of 151 species and 5 subspecies belonging to 18 genera in Gesneriaceae were estimated and reported,and 1C- value varied by almost 4. 1 fold at the level of family. According to Soltis 's classification,all available genome size for Gesneriaceae are small or very small genomes.
出处
《陇东学院学报》
2016年第1期53-58,共6页
Journal of Longdong University
基金
陇东学院博士科研启动基金(XYBY1412)